The role of citric acid cycle and related metabolites in IDH1-mutated gliomas

我们利用第三方软件LC模型对19例胶质瘤标本进行了瘤内代谢分析，该模型可以计算每种代谢物的定量MR谱值。我们特别关注柠檬酸相关的代谢物，包括N-乙酰天冬氨酸（NAA）和N-乙酰乙酰谷氨酸（NAAG）。胶质细胞内的主要代谢产物如葡萄糖、谷氨酸、GPC在IDH突变型和野生型胶质瘤之间没有显著变化。NAA也没有显示显著差异，而NAAG显示IDH突变的胶质瘤中的0.237（35%降低）与野生型胶质瘤中的0.362相比，表明胶质瘤细胞中的代谢环境在IDH突变的胶质瘤和野生型胶质瘤之间显著改变。术前应用LC模型对胶质瘤进行MR分析有可能对IDH突变胶质瘤进行鉴别诊断。

We analyzed the intratumoral metabolism of 19 glioma samples using the LC model that the third party software which can calculate quantitative MR spectrum value of each metabolites. We especially focused on citric acid related metabolites including N-acetyl-aspartate (NAA) and N-acetyl-aspartyl-glutamate (NAAG). Major metabolites in glial cells such as glucose, glutamate acid, GPC did not indicate significant change between IDH-mutated and wild-type glioma. NAA also did not indicate significant differences, whereas NAAG indicated 0.237 (35% reduction) in IDH-mutated glioma comparison to 0.362 in wild type glioma, suggesting that metabolic environment in glioma cells were significantly changed between IDH-mutated and wild-type glioma. Pre-operative MR analysis of glioma using LC model has the possibility of differential diagnosis of IDH-mutated glioma.