Development of photosensitizer with urea-Establishment of periodontal treatment combined with Er:YAG laser.

近年来，人们对牙科激光治疗牙周病的兴趣与日俱增。本研究的目的是获得一种有效的Er：YAG激光光敏剂，并评价以含尿素试剂为光敏剂的抗菌光动力疗法(APDT)对牙周组织的治疗效果。结果表明，5%尿素对牙周组织的去除效果最好。激光+光敏剂组随能量密度的增加，清除内毒素的效率提高，但激光+光敏剂组与激光+光敏剂组无显著差异。

In recent years, there has been growing interest in the use of dental lasers for treatment of periodontal diseases. The purpose of this study was to acquire an effective Er:YAG laser treatment with photosensitizer and evaluate the effects of antimicrobial photodynamic therapy(aPDT) with urea-containing reagent as photosensitizer for periodontal treatment.It was comfirmed that the concentration of urea the most effectively involved in tarter removement was 5% urea.With urea-containing reagent application, the number of P.gingivalis decreased with increasing Er:YAG laser irradiation time in vitro. Laser with photosensitizer reagent group had a higher LPS elimination efficiency with increasing energy density,there was no significant difference between laser group and laser with photosensitizer reagent group though.