Possible functions of the permeability barrier of the nuclear envelope.

我研究了在小鼠成纤维细胞瑞士3T3细胞系中观察到的高核膜通透性的现象。在本研究中，我用荧光蛋白作为通透性的探针，通过活细胞成像技术获得了以下结果。首先，高NE渗透性不是持续性的，而是暂时性的。第二，据报道，胞浆内钙离子浓度影响去甲肾上腺素的通透性，但不是Swiss3T3细胞中观察到的高去甲肾上腺素的原因。第三，这种现象不仅发生在Swiss3T3细胞系中，而且可能具有普遍性，因为在NIH3T3细胞系和HT1080细胞系中也观察到了高NE通透性。

I studied the phenomenon of the high nuclear envelope (NE) permeability observed in the mouse fibroblast, Swiss 3T3 cell line. In this research, I obtained the following results by live cell imaging techniques using fluorescent proteins as probes for the permeability. First, the high NE permeability was not persistent but transient. Second, the calcium ion concentration in the cytoplasm, which is reported to affect the NE permeability, was not the cause of the high NE observed in Swiss3T3 cells. Third, this phenomenon was not specific only to the Swiss3T3 cell line but could be general because the high NE permeability was also observed in the NIH3T3 cell line and the HT1080 cell line.