Funktion Eisen-transportierender CDF-Proteine in magnetotaktischen Bakterien und E. coli

阳离子扩散促进剂(CDF)家族的膜结合转运蛋白在原核生物中广泛存在，但这些蛋白的生理功能直到最近仍不清楚。对该家族成员的研究表明，这些蛋白可能转运Fe(II)，是细菌细胞铁稳态系统的一部分。利用分子遗传学的方法可以很容易地对E.Coli和Fief进行操作。另一方面，MAMB和MAMM是假定的铁转运体，具有不同的性质，因为磁小体生物矿化需要运输大量的铁。此外，格雷菲斯瓦尔登斯分枝杆菌最近通过基因技术变得可驯服，磁小体的形成是一种独特而明显的现象。因此，哈勒和不来梅的团队联合起来，以相互关联的背靠背方法参与CDF蛋白运输铁的竞争激烈的领域：将使用高度集成的遗传学、生化和生理学方法研究参与磁小体生物合成的FIEF和CDF样Mam-蛋白的生理功能和生化机制。最终，我们不仅希望揭示特定蛋白质在铁稳态和磁小体形成中的代谢相关性，而且我们将能够指定迄今尚未确定的CDF3蛋白的整个家族的功能。

A specific subset of proteins that belong to the cation diffusion facilitator (CDF) family of membrane-bound transporters very frequently occurs in prokaryotes, but the physiological function of these proteins has remained obscure until recently. Data obtained for members of that family, Escherichia coli (FieF, former name Yiip) and Magnetospirillum gryphiswaldense (MamB, MamM), indicate that these proteins might transport Fe(II) and be part of the cellular iron homeostasis system in bacteria. E.coli and FieF are easy to manipulate by the methods of molecular genetics. MamB and MamM, on the orther hand are putative iron transporters with unusual properties since magnetosome biomineralization requires the transport of vast amounts of iron. In addition, M. gryphiswaldense has recently become amenable by genetic techniques, and magnetosome formation is a unique and conspicuous phenomenon. Therefore, the groups in Halle and Bremen joined forces to engage into the highly competitive field of iron transport by CDF proteins in an interlinked back-to-back approach: The physiological functions and biochemical mechanism of FieF and CDF-like Mam-proteins involved in magnetosome biosynthesis will be studied using a highly intergrated genetic, biochemical and physological approach. Ultimately, we not only expect to unveil the metabolic relevance of the specified proteins in iron homeostasis and magnetosome formation, respectively, but we will be able to assign a function to the whole family of so far uncharacterized CDF3 proteins.