記憶回路を律動的に制御する機構の同定とモデル化

在过去的八个月里，Sekulic博士结合了行为、免疫组织化学和遗传方法，在小鼠身上诱导了新的记忆，并随后标记了活跃细胞，特别是参与θ波节律的中间神经元，代表了新的神经元群。他评估了新标记神经元与感兴趣的抑制性中间神经元群体的重叠，特别是O-LM细胞。使用的小鼠是野生型生长抑素- cre （SST-Cre）小鼠的混合物，用于标记O-LM细胞，cfos-tTA和cfos-tTA/SST-Cre双阳性小鼠用于标记新记忆期间可能是O-LM细胞的活跃神经元。此外，RAM （Robust Activity Marking）病毒（一种最近开发的标记新活跃神经元的工具）也与SST-Cre小鼠联合使用。这些努力在我的实验室里是一个全新的研究方向，我对他的努力和生产力非常满意。与此同时，Sekulic博士已经开始了他的体内生理记录训练，以监测自由小鼠中这些神经元群的动态活动。他已经取得了很好的进展，不久将开始收集必要的资料。

In the last eight months Dr. Sekulic combined behavioural, immunohistochemical and genetic approaches to induce new memories in mice and subsequently label active cells, specifically interneurons involved in the theta rhythm, representative of the novel neuronal ensemble. He evaluated the overlap of the newly marked neurons with the population of inhibitory interneurons of interest, specifically O-LM cells. The mice used were a mix of wild-type, Somatostatin-Cre (SST-Cre) mice for labeling O-LM cells and cfos-tTA and cfos-tTA/SST-Cre double positive mice for marking active neurons during new memories that might be O-LM cells. Additionally, the RAM (Robust Activity Marking) virus, a recently developed tool for marking newly active neurons, was also used in combination with SST-Cre mice. These efforts are an entirely new line of inquiry in my laboratory and I am very satisfied with his efforts and productivity. In parallel Dr. Sekulic has started his training in in vivo physiological recording to monitor the dynamic activity of this neuronal population in freely mice. He has made excellent progress and will soon begin to collect the necessary data.