Molecular elucidation of chromatin-level regulation in plant immunity.

我们已经完成了水稻原生质体和愈伤组织HAC701融合蛋白的构建，但从未进行过实质性的测试。当预算可用于该项目时，这将继续进行。我们已经产生了hac701/wrky45双突变体，并将准备在植物表观遗传学单位(OIST)的帮助下进行基因分型。这个双重突变体将澄清评论家关于CRISPR/Cas9靶向用于沉默HAC701的特异性的一些问题。综上所述，我们为HAC701和WRKY45的遗传互作提供了证据。我们还证实了H3K9ac可能是由HAC701乙酰转移酶催化的。最后，我们在H3K9ac组分中发现了WRKY45序列的丰富，为HAC701与WRKY45位点可能的物理相互作用提供了更多的证据。如果提供重大修改，该手稿已被接受在通信生物学杂志上发表。

We have finished the construction of HAC701 fusion protein for rice protoplast and callus, but was never substantially tested. This will be continued as budget becomes available for this project. We have produced the hac701/wrky45 double mutant and will be ready for genotyping with the help from Plant Epigenetics Unit (OIST). This double mutant will clarify some of the reviewer’s questions on the specificity of CRISPR/Cas9 targeting used to silence HAC701. In summary, we have provided evidence for the genetic interaction of HAC701 and WRKY45. Also, we have confirmed that H3K9ac is possibly catalyzed by HAC701 acetyltransferase. Lastly, we found enrichment of WRKY45 sequences in H3K9ac fraction adding more evidence of the possible physical interaction of HAC701 with WRKY45 locus. The manuscript has been accepted for publication at Communications Biology provided major revisions be supplied.