Impact of Land-use on Biogeographical Diversity of Soil Prokaryotes and their Functional Links to Atmospheric Chemistry

将研究与大气化学有关的原核生物群亚群的功能生物多样性和差异调节。这项研究将探讨“什么决定一个人得到什么”的问题。通过全面的综合实验设计，评估对比土地利用下的土壤。目的是（a）通过互补培养和功能基因分析来评估固氮菌和甲基营养菌的活性，（B）确定土地利用对固氮菌和甲基营养菌活性的影响，（c）通过mRNA分析和稳定同位素探测来鉴定活性固氮菌和甲基营养菌，（d）鉴定新的基因型，（e）解决群落结构的时间/空间变化，并开发诊断功能基因微阵列，以及（f）建立一个基于功能基因的“生态型概念”，用于微生物和甲基营养生物。另一项成果将是改进评估这些职能组的方法。通过将功能多样性和活动数据与生物多样性勘探站的原位土壤参数数据库相关联，这项工作还将增加我们对原核生物多样性如何影响生态系统功能的理解，并深入了解可能驱动原核生物物种形成的因素。

The functional biodiversity and differential regulation of subpopulations of prokaryotic biomes associated with atmospheric chemistry will be investigated. The study will address the question “What determines what one gets?” with a comprehensive, integrative experimental design that will evaluate soils under contrasting land-use. The objectives are to (a) assess denitrifier and methylotroph diversities by complementary cultivation and functional gene analyses, (b) determine the effect of land-use on activities of denitrifiers and methylotrophs, (c) identify active denitrifiers and methylotrophs by mRNA analyses and stable isotope probing, (d) identify novel genotypes, (e) resolve temporal/spatial variations in community structures and develop a diagnostic functional gene microarray, and (f) establish a functional gene-based ‘ecotype concept’ for denitrifiers and methylotrophs. An additional outcome will be the improvement of methods for the assessment of these functional groups. By correlating data on functional diversity and activity with the database on in situ soil parameters of the biodiversity exploratories, this work will also increase our understanding of how prokaryotic diversity impacts on ecosystem function and provide insight into factors that might drive prokaryotic speciation.