Molecular analysis of T cell antigen receptors and functional molecules.

本文利用BW 5147与C57 BL/6 KLH-Ts融合的可诱导KLH特异性抗IdTs杂交瘤细胞株，分析了抑制性T细胞（Ts）上抗独特型（Id）受体的免疫学作用及其分子遗传学特性。得出以下观察结果和结论。抗Id Ts受体作为外部抗原的调节性内部图像（立体复制），主要由抑制基因家族看到。通过以下四个实验获得了内部图像由Ts受体V区组成的证据：a）抗Id Ts杂交瘤在细胞表面表达外部抗原表位（KLH样决定簇）的立体拷贝; B）特定的抗KLH抗体与立体拷贝反应，并且还具有以KLH特异性和H- <2 B>限制性方式抑制应答的活性; c）Ts上KLH样决定簇的表型表达在嵌合条件下发生改变; d）抗Id Ts杂交瘤的大鼠抗体与KLH和KLH特异性反应，并且介导H- <2 B>限制性抑制活性。用X射线照射骨髓嵌合体解决了I-J悖论。I-J决定簇被认为是Ts上的独特型结构。用FPLC系统分析了抗IdTs杂交瘤中的抑制因子，发现其分子量为30 KD，等电点为6.1-6.2.5。我们还成功地克隆和测序了Ts α链基因（cDNA），该基因在Ts杂交瘤中具有活性。然而，Ts受体分子似乎与Th和Tc上的分子具有不同的结合，因为Ts杂交瘤中的Ts β链基因全部缺失。提出了一种与Ts α链相伴存在的未定义的第四链。

We attempted to analyse immunological roles of anti-idiotypic (Id) receptor on suppressor T cells (Ts) and their molecular genetic properties by using inducible KLH specific anti-Id Ts hybridoma make by fusion of BW5147 and C57BL/6 KLH-Ts. The following observations and conclusions were oftained.1. Anti-Id Ts receptor works as an regulatory internal image (stereocopy) of the external antigen predominantly seen by suppressor families.2. The evidence that the internal image is composed of the Ts receptor V region is obtained by the following four experiments:a) Anti-Id Ts hybridoma expresses the stereocopy of the external antigenic epitope (KLH-like determinant) on the cell surface.b) The particular anti-KLH antibody reacts with the stereocopy and also possesses the activity to suppress the responses in the KLH specific and H- <2^b> restricted manner.c) The phenotypic expression of the KLH-like determinant on Ts is changed under chimera conditions.d) The rat antibody against anti-Id Ts hybridoma reacts with KLH and KLH-specific and mediated H- <2^b> restricted suppressor activity.3. The paradox of I-J has been solved by using x-irradiation bone marrow chimera. The I-J determinant is found to be the idiotypic structure on Ts.4. The suppressor factor derived from anti-Id Ts hybridoma is analysed by FPLC system and found to be the 30 KD molecule with pI 6.1-6.2.5. We have also been successful in cloning and sequencing of Ts alpha chain gene (cDNA) which is found to be active in Ts hybridomas. However, Ts receptor molecules seem to have different configulation from those on Th and Tc, because Ts beta chain genes in Ts hybridomas are all deleted. An undefined forth chain is proposed to exist in association with Ts alpha chain.