SIGNIFICANCE OF 2-OXOALDEHYDE IN FOOD AND BIOLOGICAL SYSTEMS

1997年：(1)在溶菌酶-葡萄糖(LG)和溶菌酶-果糖(LF)反应体系中检测到葡萄糖(GLCO)、3-脱氧葡萄糖(3DG)、乙二醛(GO)和甲基乙二醛(MG)。过渡金属对GLCO和GO的生成有显著影响，而氧对3DG的生成并不是必需的。(2)通过3DG与N-苯甲酰精氨酰胺的反应，确定了新的咪唑类化合物。1998年：(1)测定了LG、LE和L-3DG反应体系中赖氨酰-吡咯吡啶和戊糖苷的含量。在3DG-溶菌酶反应体系中，赖氨酰-吡咯吡啶的量比戊糖苷多。在LG和LF反应体系中，与赖氨酰吡啶的生成量相比，戊苷的生成量增加。(2)3DG很容易与核苷中的2‘-脱氧鸟苷反应。从3DG和2‘-脱氧鸟苷的反应混合物中分离纯化了两个主要产物(G-A和G-B)。G-A鉴定为N-(1-氧代-2，4，5，6-羟基己基)-2‘-脱氧鸟苷。G-B为G-A的非对映异构体。

1997 :(1) Glucosone (GLCO), 3-deoxyglucosone (3DG), glyoxal (GO), and methyiglyoxal (MG) were detected in the reaction systems of lysozyme-glucose (LG) and lysozyme-fructose (LF). Transition metals gave significant effects on the formation of GLCO and GO.On the other hand, oxygen was not essential for the generation of 3DG.(2) Novel imidazole compound was identified by the reaction of 3DG and N -benzoylarginine amide. Lysyl-pyrropyridine was identified in the reacton of lysozyme and reducing sugars.1998 :(1) The amounts of lysyl-pyrropyridine and pentosidine were determined in LG, LE, and L-3DG reaction systems. Lysyl-pyrropyridine increased in quantity compared to pentosidine in 3DG-lysozyme reacton systems. In LG and LF reaction systems, pentosidine increased in comparison with the formation amounts of lysyl-pyrropyridine.(2) 3DG reacted readily with 2'-deoxyguanosine in nucleosides. Two major products (G-A and G-B) were isolated and purified from the reaction mixture of 3DG and 2'-deoxyguariosine. G-A was identified as N- (1-oxo-2,4,5,6-hydroxyhexyl) -2'- deoxyguanosine. G-B was identified as a diastereomer of G-A.