Elucidation of correlation between structure and function of glyceraldehyde modified products

甘油醛改性产物结构与功能相关性的阐明

• 批准号: 22580149
• 负责人: HAYASE Fumitaka
• 金额: $ 2.91万
• 依托单位: Meiji University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580149/
• 关键词: メイラード反応; グリセルアルデヒド; AGE; リジン; アルギニン; 糖尿病; Maillard reaction; glyceraldehyde; diabetes; lysine; ariginine; arigininine

P4 was identified as a novel dihydropyridinium compound formed by two molecules of glyceraldehyde (GLA), lysine, and arginine. The amount of P4 in serum protein, collagen, and lens proteins significantly increased compared with those in control subjects. The sequence analysis of GLA modified lysozyme revealed that K33, K97, and K116 were modified with GLA, and glyceraldehydes derived pyridinium-type AGE (GLAP) was generated in their lysine residues. Moreover, MG-H1 was also identified in arginine residues. Similar tendency was observed in GLA modified ribonuclease A and cytochrome c. The results of immunized mice with antigen caproyl-GLAP showed that GLAP polyclonal antibody which recognized the pyridinium skeleton of GLAP structure was obtained. The addition of GLA-BSA inhibited the cell proliferation of PC12, HL-60, and differentiated PC12 cells. The inhibition was dose dependent and was restored by the addition of anti-GLAP antibody. The findings were supported that the chemial structure of the epitope showing the physiological effects of GLA modified protein is GLAP.

P4是由甘油醛（GLA）、赖氨酸和精氨酸组成的新型二氢吡啶化合物。与对照组相比，血清蛋白、胶原和透镜蛋白中的P4含量显著增加。序列分析表明，K33、K97和K116经GLA修饰后，其赖氨酸残基上产生了甘油醛衍生的吡啶鎓型AGE（Glyceraldehyde derived pyridinium-type AGE，Glyceraldehyde）。此外，MG-H1也被鉴定为精氨酸残基。在GLA修饰的核糖核酸酶A和细胞色素c中观察到类似的趋势。用己酰甘精肽抗原免疫小鼠，获得了识别甘精肽吡啶骨架的多克隆抗体。GLA-BSA对PC 12、HL-60及分化的PC 12细胞的增殖均有抑制作用。抑制作用呈剂量依赖性，并可通过加入抗甘精胰岛素抗体恢复。这些结果支持了GLA修饰蛋白的生理效应表位的化学结构为Glucose。

项目成果

Formation of Maillard reaction products derived from 3-deoxyxylosone

3-脱氧木酮衍生的美拉德反应产物的形成

• 发表时间: 2012
• 作者: T. Usui;H. Watanabe;F. Hayase et.al.
• 通讯作者: F. Hayase et.al.

グリセ ルアルデヒドとクレアチン、リジンの共存下 で生成するメイラード反応生成物の同定

甘油醛、肌酸、赖氨酸共存时产生的美拉德反应产物的鉴定

• 发表时间: 2010
• 作者: 臼井照幸; 渡辺寛人;早瀬文孝 ら
• 通讯作者: 早瀬文孝 ら

グアニ ジノ化合物によるグリセルアルデヒド由来 グリケーション生成物の形成抑制

胍基化合物抑制甘油醛衍生的糖化产物的形成

• 发表时间: 2012
• 作者: 木村圭一;渡辺寛人; 早瀬 孝 ら
• 通讯作者: 早瀬 孝 ら

グアニジノ化合物によるグリセルアルデヒド由来グリケーション生成物の形成抑制

胍基化合物抑制甘油醛衍生的糖化产物的形成

• 发表时间: 2012
• 作者: 木村圭一;臼井照幸;渡辺寛人;早瀬文孝
• 通讯作者: 早瀬文孝

糖尿病におけるグリセルアルデヒド由来蛍光性架 橋グリケーション生成物の分析

糖尿病中甘油醛衍生荧光交联糖化产物的分析

• 发表时间: 2011
• 作者: 森澤秀行; 渡辺寛人;早瀬文孝 ら
• 通讯作者: 早瀬文孝 ら