Development of anti-disease foodstuffs by use of a new radical scavenging system, and their verification and application in vivo

XYZ体系中Y组分的抗氧化活性与化学发光强度的关系，其中X、Y、Z是活性氧种、抗氧化剂(催化种)和受体。还从食品和生物系统中寻找了具有Z种作用的化合物。此外，还研究了Y的化学发光强度与其体内抗氧化活性的关系。另一方面，被发现具有抑制癌症作用的Y或Z的活性被确定，当这些成分单独使用或与有助于预防人类乳腺癌的药物联合使用时，是否对乳腺癌细胞具有抗增殖活性。研究结果如下：1.研究结果。在脂质过氧化体系中表现出较强抗氧化活性的Y组分，在XYZ体系中也表现出较强的化学发光。这些结果表明，XYZ系统可用于食品中抗氧化物质的检测。(-)-表没食子儿茶素没食子酸酯(ECG)有效地防止了百草枯诱导的大鼠氧化应激，与(-)-表儿茶素相比，(-)-表儿茶素的化学发光比鸡蛋弱。这一结果也表明，XYZ系统可以有效地检测Y，从而有效地防止体内的氧化应激。血红蛋白、细胞色素C、过氧化物酶、超氧化物歧化酶。过氧化氢酶在体外具有较强的诱导脂质过氧化作用，在存在典型的Y组分的情况下，表现为典型的Z组分。MISO中的MISO和Bichanin A单独或与预防乳腺癌的药物联合应用于大鼠移植的乳腺癌细胞时显示出抑制增殖的作用。

The relationship between antioxidative activity of Y component in XYZ system and intensity of chemiluminescence, in which X, Y and Z are active oxygen species, antioxidants (catalytic species) and receptors. Compounds act as Z species were also searched from foodstuffs and biological system. In addition the relationship between intensity of chemiluminescence of Y and its antioxidative activity in vivo were examined. On the other hand, activity of Y or Z, which was found to act to suppress cancer, was determined whether these components show antiproliferative activity for mammary cancer cell when were used alone or in combination with drug useful for prevention human breast cancer. The results were as follows.1. Y component which shows strong antioxidative activity in lipid peroxidation system, showed strong chemiluminescence in XYZ system, too. These results showed that XYZ system are useful to determine antioxidative substances from foodstuffs.2. (-)-Epicatechin gallate (ECg) prevented effectively paraquat-induced oxidative stress in rats when compared to the effect of (-)-epicatechin, which shows weak chemiluminescence than EGg. This result also showed that XYZ system is useful to examine Y which is effective to prevent oxidative stress in vivo.3. Hemoglobin, cytochrome C, peroxidase, superoxide dismutase. and catalase which are rather induce lipid peroxidation in vitro, acted as typical Z component in the presence of typical Y component.4. Miso and biochanin A in miso demonstrated antiproliferative effect when they were given to rats trasnplanted mammary cancer cells alone or in combination with drug useful for mammary cancer prevention.