STUDIES ON CONTROL OF ENZYME REANTIONS PERFORMED BY SUPRAMOLECULAR NANO-REACTORS

1.结合人工受体、酶和双层形成脂质，构建了作为能够控制酶活性的纳米反应器的超分子组装体.在极性头部和疏水性双链段之间插入氨基酸残基的合成肽脂质形成了形态稳定的双层囊泡作为纳米级反应容器. NADH依赖性乳酸脱氢酶主要通过静电相互作用与囊泡表面结合，在水介质中保持活性位点的构象.由三个功能组分组成的类固醇环番，环番环，四个胆汁酸部分，和连接它们的四个赖氨酸残基，被制备为人工受体。当受体包埋在双层膜中时，该分子表现为纳米反应器的分子开关，进行双信号传递.具有氨基的胆汁酸衍生物作为人工细胞表面受体，能够通过在合成双层膜中进行双重信号识别和相重组来激活乳酸脱氢酶。对于具有偶氮苯部分的人工跨膜受体也观察到类似的行为。芳香醛，如1 -羟基-2-萘甲醛和铜（II）离子是有效的外部和第二信使信号，分别为这些人工受体。

1. Supramolecular assemblies as nano-reactors being capable of controlling enzymic activity were constituted in combination with an artificial receptor, an enzyme, and a bilayer-forming lipid.2. Synthetic peptide lipids having an amino acid residue interposed between a polar head moiety and a hydrophobic double-chain segment formed morphologically stable bilayer vesicles as nano-scale reaction vessels.3. NADH-dependent lactate dehydrogenase was bound to the vesicular surface, mainly through electrostatic interactions, keeping a conformation of the active site in aqueous media.4. The steroid cyclophane composed of three functional components, a cyclophane ring, four bile acid moieties, and four lysine residues connecting them, was prepared as an artificial receptor. When the receptor was embedded in the bilayer membrane, this molecule behaved as a molecular switch of the nano-reactor by performing double signal recognition.5. A bile acid derivative having an amino group acted as an artificial cell-surface receptor capable of activating lactate dehydrogenase by performing double signal recognition and phase reorganization in a synthetic bilayer membrane. Analogous behavior was also observed for an artificial transmembrane receptor having an azobenzene moiety.6. Aromatic aldehydes such as 1 -hydroxy-2-naphthaldehyde and cupper(II) ions were effective external and second messenger signals, respectively, for these artificial receptors.