The Chromium Connect, an integrated and robotic system to automate library preparation for single-cell RNA-Seq

项目总结/摘要 我们建议收购Chromium Connect系统。拟议的文书将 被安排在斯坦福大学基因组学和个性化医学中心的一个共享环境中， （SCGPM）测序服务中心，由一组训练有素的经验丰富的工作人员操作 基因组技术。SCGPM测序服务中心是核心设施， 成立于2009年，对整个斯坦福大学社区开放。自成立以来，SCGPM 测序服务中心已经为超过700名斯坦福大学的研究人员提供了测序数据 150多个实验室。单细胞测序已成为一种变革性工具 在生物医学研究领域和测序成本的快速下降已经产生了 需要一致和可靠的高通量文库制备。的存在 Chromium Connect还将大大提高该中心的库准备能力 缩短了准备文库的周转时间。本报告中突出显示的主要用户和次要用户 建议的范围从初级教师谁是刚刚开始自己的实验室，以建立完整的 拥有30多年研究经验的教授。几乎所有的用户都有NIH的资助， 他们的基因组研究依赖于负担得起的和可定制的超高通量， 代测序技术。我们预测，使用Chromium Connect系统将 由于高需求和对具有成本效益的图书馆的需求， 准备.通过用所提出的仪器制备的测序文库生成的数据将 积极影响人类疾病研究的广泛领域，特别是：心血管 疾病，肺动脉高压，2型糖尿病，炎症性肠病， 发育缺陷、癌症、睡眠障碍、肥胖和衰老。该研究在 这些研究将有助于更好地解释人类基因组， 将新的检测方法和技术应用于临床。斯坦福大学的研究人员 通过在该仪器上进行文库制备，可以进行单细胞测序测定 不断发表高调的研究，表明这项新技术将产生的重大影响， 在生物医学研究中的作用。

PROJECT SUMMARY/ABSTRACT We are proposing the acquisition of a Chromium Connect System. The proposed instrument will be placed in a shared setting at the Stanford Center for Genomics and Personalized Medicine (SCGPM) Sequencing Service Center and operated by a highly trained group of staff experienced in genomic technologies. The SCGPM Sequencing Service Center is a core facility that was founded in 2009 and is open to the entire Stanford community. Since its inception the SCGPM Sequencing Service Center has produced sequencing data for over 700 Stanford researchers and more than 150 laboratory groups. Single cell sequencing has become a transformative tool in the biomedical research space and the rapid decline in the cost of sequencing has generated a need for consistent and reliable high-throughput library preparation. The presence of a Chromium Connect will greatly increase the library preparation capabilities of the center as well as shorten turnaround times for preparing libraries. The Major and Minor Users highlighted in this proposal range from junior faculty who are just starting their own laboratories to established full professors with over 30 years of research experience. Nearly all of the users have NIH funding, and their genomic studies depend on affordable and customizable ultra high-throughput, next generation sequencing technologies. We predict that use of the Chromium Connect System will approach maximum capacity due to the high demand and need for cost-effective library preparation. Data generated by sequencing libraries prepared with the proposed instrument will positively impact a broad cross section of human disease research, notably: cardiovascular disease, pulmonary hypertension, type 2 diabetes mellitus, inflammatory bowel diseases, developmental defects, cancer, sleep disorders, obesity, and aging. The research performed in these studies will help pave the way for better interpretations of the human genome as well as the translation of new assays and technologies to the clinic. Stanford researchers who perform single cell sequencing assays made possible by library preparation performed on this instrument consistently publish high profile studies, indicating the major impact that this new technology will have on biomedical research.