Organ Specific Project
Organ Specific Project
批准号:
10531083
负责人:
MICHAEL P. SNYDER
金额:
$156.45万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2022
资助国家:
美国
项目状态:
未结题
起止时间:
2022-09-23 至 2026-06-30
关键词:
3-DimensionalATAC-seqAmerican IndiansAntibodiesArchitectureAreaBiological AssayBlood VesselsBrain DeathCOVID-19 testingCell NucleusCellsChromatinColonCommunitiesComplexConfounding Factors (Epidemiology)ConsentConsent FormsDataData SetDiseaseDistal part of ileumDuodenumEnrollmentEnterocytesExhibitsFluorescent in Situ HybridizationFreezingFunctional disorderGastrointestinal tract structureGene ExpressionGenerationsGenesGeographyGoalsHumanHuman BioMolecular Atlas ProgramImmuneImmune systemImmunoassayIndividualInflammatory Bowel DiseasesIntestinesInvestigationIronLeadLengthLightLymphaticMalignant NeoplasmsMapsMetabolismMolecularMonitorMucous MembraneMuscleNerveNeurophysiology - biologic functionNuclearNutrientOrganOrgan DonorOrgan ProcurementsOrgan TransplantationPhasePhysiologicalPhysiologyPoliciesPopulationPositioning AttributePrecision HealthProductionProtocols documentationRNAResolutionResourcesRiboflavinSamplingSiteSmall IntestinesSouth DakotaStandardizationStructureSurfaceSystemTechniquesThiamineThickTissue PreservationTissuesTranscriptTransplant SurgeonVitamin B 12Vitamin KWorkbile saltsbody systemcell typecommensal microbesethnic diversityhuman tissueimprovedlipidomicsmetabolomicsmicrobiomemultimodal datamultimodalitynutrient absorptionprogramsreconstructionspatial relationshiptissue mappingtranscriptometranscriptome sequencingtranscriptomicstwo-dimensionalwhole genomeworking group
中文摘要
摘要:器官特异性项目
除了营养吸收之外,胃肠道对正常和
其他器官系统的疾病生理学,包括新陈代谢,神经功能,
免疫系统一个高分辨率,多模式的肠道3D地图将是一个非常宝贵的
了解正常肠道功能和导致疾病的扰动的资源。我们
我们的目标是沿着小肠和结肠的长度沿着创建这个地图,这两个地图都
在功能上有着细微的地理分工。使用单核RNA+ATAC-seq,我们
将同时分析基因表达和定义复合物的调控程序,
驱动肠道功能的细胞群。我们将使用CODEX多重免疫测定,
定义这些细胞群的空间关系,并使用序列
路段我们还将使用分子制图多重FISH测定来生成
转录物空间图,以更好地将单核数据整合到CODEX免疫图中。
这些研究将在保存和获取的组织上进行,
适用于高质量的分子图谱生成。我们的目标里程碑是描述六个
在完全覆盖下,来自总共8个人口统计学上不同的个体的肠道部位。完成网站
在更多的个体中绘制更深的覆盖范围,
20个人。
英文摘要
ABSTRACT: Organ Specific Project
Beyond nutrient absorption, the gastrointestinal tract has wide-ranging effects on the normal and
diseased physiologies of other organ systems, including metabolism, neural function, and the
immune system. A high-resolution, multi-modal 3D map of the bowel would be an invaluable
resource to understand normal bowel function and the perturbations that lead to disease. Our
continued goal is to create this map along the length of the small bowel and colon, both of which
have nuanced geographic specializations of function. Using single-nuclei RNA+ATAC-seq, we
will simultaneously profile the gene expression and regulatory programs that define the complex
cell populations that drive bowel function. We will use the CODEX multiplex immunoassay to
define the spatial relationships of these cell populations and to create 3D maps using serial
sections. We will also use the Molecular Cartography multiplex FISH assay to generate
transcript spatial maps to better integrate the single-nuclei data to the CODEX immuno-maps.
These investigations will be performed on tissues that are preserved and procured in a manner
suitable for high-quality molecular map generation. Our target milestone is to characterize six
bowel sites from a total of 8 demographically-diverse individuals at full coverage. To generate
deeper coverage maps in more individuals, two bowel sites will be mapped at light coverage in
20 individuals.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
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Multi-Modal Wireless COVID Monitoring & Infection Alerts for Concentrated Populations
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Multi-Modal Wireless COVID Monitoring & Infection Alerts for Concentrated Populations
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资助金额:$28.66万
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财政年份:2019
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负责人:MICHAEL P. SNYDER
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依托单位:
Genomics Diversity Summer Program (GDSP) at Stanford
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批准号:10162634
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项目类别:
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资助金额:$14.3万
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财政年份:2019
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负责人:MICHAEL P. SNYDER
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依托单位:
Genomics Diversity Summer Program (GDSP) at Stanford
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负责人:MICHAEL P. SNYDER
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依托单位:
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财政年份:2018
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负责人:MICHAEL P. SNYDER
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依托单位:
Stanford Tissue Mapping Center
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批准号:10213801
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资助金额:$20.45万
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财政年份:2018
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负责人:MICHAEL P. SNYDER
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依托单位:
Multiomic Signatures of Microbial Metabolites Following Prebiotic Fiber Supplementation
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批准号:9788264
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项目类别:
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资助金额:$39.25万
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财政年份:2018
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负责人:MICHAEL P. SNYDER
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依托单位:
PRODUCTION CENTER FOR MAPPING REGULATORY REGIONS OF THE HUMAN GENOME
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依托单位:
High-throughput sequencer for multi-scale genomic studies
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依托单位:
Core B: Bioinformatics Core
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依托单位:
国内基金
海外基金
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