Upgrade the 14T Ultrahigh Field Horizontal MR Scanner for Rodent and ex-vivo Imaging

这项提议的目标是购买一台布鲁克AV-Neo游戏机来取代过时的 西门子14T卧式磁共振扫描仪(内径13厘米)的控制台，已专门用于 支持动物和动物的高分辨率解剖和功能动态脑图 体外人脑组织成像。马萨诸塞州综合医院的马提诺斯中心 一直站在开发高级功能映射方法的前沿，例如Functive MRI，并实施尖端的MRI方法，以架起基础和翻译 学习。追求新一代尖端成像方法，为 更高领域的MRI翻译研究，迫切需要改进我们的14T MRI临床前 用于高分辨率动物和体外成像的平台。特别是，拟议的14T MR 控制台升级将提升新方法的翻译潜力，例如单一- 血管功能磁共振成像和行扫描功能磁共振成像，架起细胞和微血管解剖和功能的桥梁 从动物到人脑的动力学。此外，这项建议还将支持20多个研究项目 由美国国立卫生研究院资助，在脑机制研究方面做出批判性的翻译努力 疾病包括阿尔茨海默病(AD)、帕金森氏病、脑血管性痴呆、 偏头痛、创伤性脑损伤和心脏骤停(AC)引起的昏迷，以及 肝炎和肝纤维化动物模型的器官成像。高度协同的 我们的提案中概述的合作研究项目可以概括为三个主要方面 主题：1)神经血管动态信号，2)前沿神经成像方法，3) 动物疾病模型的多模式机械特征。我们已经建立了14T- 基于多模式成像平台的高分辨率解剖与功能MRI相结合 利用新兴的神经技术进行成像，如光遗传学、光纤介导式生物传感器 记录钙、谷氨酸等，促进对 大脑功能的复杂性。布鲁克AV-NEO系统将提供关键的技术创新 为了提高新的脑成像方法的性能，例如单血管功能磁共振成像，LINE- 基于最先进的电子学，扫描功能磁共振成像，射频平板特定扩散加权成像 和软件设计。因此，拟议的仪器升级不仅将加速 所列项目的进展情况，但也促进了最新MR方法的翻译 为小灵通资助的调查人员提供真正的多学科、地区性资源。

The goal of this proposal is to purchase a Bruker AV-Neo console to replace the out-of-date Siemens console of the 14T horizontal MR scanner (13cm bore), which has been dedicated to supporting the high-resolution anatomical and functional dynamic brain mapping of animals and ex-vivo human brain tissue imaging. The Martinos Center at the Massachusetts General Hospital has been at the forefront of developing advanced functional mapping methods, e.g., functional MRI, and implementing the cutting-edge MRI methods to bridge the basic and translational studies. To pursue the next-generation cutting-edge imaging methodology and prepare for the higher field MRI translational studies, there is an urgent need to improve our 14T MRI preclinical platform for high-resolution animal and ex vivo imaging. In particular, the proposed 14T MR console upgrade will boost the translational potentials of the novel methodology, e.g. the single- vessel fMRI and line-scanning fMRI, to bridge cellular and microvascular anatomy and functional dynamics from animal to human brains. Also, this proposal will support over 20 research projects funded by NIH, presenting critical translational efforts on the mechanistic studies of brain disorders including Alzheimer’s Diseases (AD), Parkinson’s Disease, cerebrovascular dementia, migraine, traumatic brain injury, and cardiac arrest (AC)-induced coma, as well as for the internal organ imaging of animal models with hepatitis and hepatic fibrogenesis. The highly synergistic and collaborative research projects outlined in our proposal can be summarized in three main themes: 1) Neurovascular dynamic signaling, 2) Cutting-edge neuroimaging methodology, 3) Multimodal mechanistic signatures of animal disease models. We have established the 14T- based multi-modal imaging platform to combine the high-resolution anatomical and functional MRI imaging with the emerging neuro-techniques, e.g. optogenetics, optical fiber-mediated biosensor recording of Calcium, Glutamate, etc, promoting novel mechanistic understanding of the complexity of brain function. The Bruker AV-Neo system will provide key technological innovations to improve the performance of the novel brain mapping methods, e.g. the single-vessel fMRI, line- scanning fMRI, RF slab-specific diffusion-weighted MRI, based on the state-of-the-art electronics and software design. Therefore, the proposed instrument upgrade would not only accelerate the progress of the listed projects but also facilitate the translation of cutting-edge MR methodologies as a truly multidisciplinary, regional resource for PHS funded investigators.