Wyatt SEC-MALS system

项目总结/摘要 卫生保健研究从来没有比在全球危机期间更重要。在公认的X光 宾夕法尼亚州立大学（宾夕法尼亚州立大学）的晶体学设施，我们寻求资金来取代我们的 过时的Viscotek 802动态光散射（DLS）设备与现代化的Wyatt Technology DLS， 多角度光散射（MALS）设备与尺寸排阻色谱（SEC）串联。这将 能够对许多疾病相关的生物分子提供准确的大小、分子量和化学计量数据， 病毒和细菌蛋白，马达、核小体和内在无序蛋白，核酸， 脂质体及其复合物。Viscotek 802已经作为主力机使用了超过15年， 我们很好。升级到现代SEC-MALS能力，我们将胜任地满足生物物理和 我们的许多特殊的NIH资助的教师的结构表征的需求，并得到设施更接近 一种综合的结构生物学方法。我们已经确定怀亚特组件是唯一的商业 符合我们要求的产品是最先进的，具有成本效益。怀亚特DLS和SEC- MALS系统具有精度高、分辨率好、灵敏度上级等优点，是目前国内最重要的 成功的17个尖端卫生保健NIH项目中描述的建议-（a）蛋白质 在汉考克、Weinert、Yengo、Boehr、Cotruvo和Llinas labs（B）蛋白复合物中的低聚物 Tan，Krasilnikov和Chroneos实验室（c）贝维拉夸实验室的RNA（d） Showalter和Zhang实验室和（e）Tian实验室的脂质体。跟踪SEC-MALS运行的能力 与内部小角X射线散射（SAXS）运行在同一样品是一个令人兴奋的和新颖的 配置. MALS-SAXS设置设计得当，具有Wyatt SEC-MALS系统的输出端口 与理学生物SAXS的输入相匹配通过这次握手，我们将投资于真正的协同效应， SEC-MALS的实验结果将指导SAXS和低分辨率的解释 从SAXS获得的溶剂包封模型可以帮助晶体学和CryoEM研究， 更高的分辨率。SEC-MALS仪器将由来自两个国家的13名结构生物学教师使用。 宾夕法尼亚州立大学校园，大学公园和好时，以及三所学院，医学院，埃伯利 理学院和工学院。没有其他功能性SEC-MALS设备， 我们二十四个校园中的任何一个实验室。凭借最先进的Wyatt SEC-MALS系统，我们的目标是 在广泛的应用中推导出精确的尺寸、分子量和化学计量， 增强宾夕法尼亚州立大学X射线晶体学设备的能力。

Project Summary/Abstract Health care research has never been more critical than during a global crisis. At the well-established X-ray crystallography facility at The Pennsylvania State University (Penn State), we seek funds to replace our outdated Viscotek802 dynamic light scattering (DLS) equipment with a modern, Wyatt Technology DLS, multi angle light scattering (MALS) equipment in-line with size exclusion chromatography (SEC). This will enable accurate size, molecular weight, and stoichiometry data on a host of disease related biomolecules, viral and bacterial proteins, motor, nucleosome and intrinsically disordered proteins, nucleic acids, liposomes and their complexes. The Viscotek802 has been a workhorse for over fifteen years and served us well. Upgrading to a modern SEC-MALS capability, we will cater competently to the biophysical and structural characterization needs of our many exceptional NIH funded faculty and get the facility closer to an integrated structural biology approach. We have identified the Wyatt components as the sole commercial product which meets our requirements, is state-of-the-art and cost-effective. The Wyatt DLS and SEC- MALS system has a high accuracy, good resolution and superior sensitivity and is paramount for the success of the seventeen cutting edge health care NIH projects described in this proposal- (a) protein oligomers in the Hancock, Weinert, Yengo, Boehr, Cotruvo and Llinas labs (b) protein complexes in the Tan, Krasilnikov and Chroneos labs (c) RNA in the Bevilacqua lab (d) intrinsically disordered proteins in the Showalter and Zhang labs and (e) liposomes in the Tian lab. The ability to follow up the SEC-MALS run with an in-house small angle X-ray scattering (SAXS) run on the same sample is an exciting and novel configuration. The MALS-SAXS setup is designed aptly, with the output port of the Wyatt SEC-MALS system matching the input of the Rigaku-bioSAXS. With this handshake, we would be investing in true synergy where experimental results from SEC-MALS would guide the interpretation of SAXS and low-resolution solvent envelope models as obtained from SAXS could help in the crystallographic and CryoEM studies at higher resolution. The SEC-MALS instrument will be used by thirteen structural biology faculty from two Penn State campuses, University Park and Hershey, and three colleges, College of Medicine, Eberly College of Science, and the College of Engineering. No other functional SEC-MALS equipment exists in any lab on any of our twenty-four campuses. With a state-of-the-art Wyatt SEC-MALS system, our goal is to deduce accurate size, molecular weight, and stoichiometry in a wide spectrum of applications and enhance the capability at the Penn State University X-ray crystallography facility.