P30 - Core Grant for Vision Research

项目摘要 这个NEI视觉研究中心核心补助金申请的广泛目标是促进研究 视觉系统在健康和致盲疾病中的结构、发育和功能， 预防、减轻或治愈这些疾病，或恢复丧失的视力， 最先进的技术。四个资源和服务核心将有助于实现广泛的 目标如下： I.图像分析和图形核心：形态分析;计算机辅助图像分析; 制作用于数据分析、介绍和出版的图形 二.形态学核心：组织学（石蜡和塑料包埋，切片和染色，冷冻 切片用于免疫组织化学）、眼部成像（裂隙灯检查和摄影，Micron III 眼底照相（荧光素血管造影）和光学相干断层扫描（OCT），生物特征 Bioptigen OCT）和视觉功能测试（ERG、OptoMotry和IOP）以及体内啮齿动物眼睛注射 电穿孔仪设置。显微镜检查（光学显微镜-明视野、暗视野、相差、DIC和 荧光）和显微摄影，以及先进的显微镜使用旋转盘和 共焦显微镜）。 三.计算机/IT核心：为定制研究需求编程;协助计算机和 信息技术硬件和软件的选择、安装、使用说明、维护和次要 维修. 四.快速原型和设计核心：设计新的独特的研究设备加上教育 和培训用户，使他们能够参与制造，建设，维护， 更新他们独特的研究设备设计。 这是一个应用程序的NEI中心核心补助金的视觉研究竞争更新提交的 首席研究员和其他19名视觉科学家，他们持有23项NEI R01研究基金。在 此外，由NEI Vision Core Grant支持的UCSF视力研究社区包括14个NEI- 支持科学家使用除现行R01以外的资助机制，4人使用其他NIH资助，1人使用FDA R01 5、私人融资。总体而言，有41名核心研究者参与了积极的研究项目， 每一个都使用至少一个中等或广泛水平的核心。利用传统和创新方法， 这项核心愿景研究资助计划非常成功，有助于提高生产力 和视觉研究的影响，吸引科学家进行视觉研究，并促进 加州大学旧金山分校的视觉系统

PROJECT SUMMARY The broad objective of this NEI Center Core Grant for Vision Research application is to facilitate study of the structure, development and function of the visual system in health and in blinding diseases, with the aim of preventing, mitigating or curing such diseases, or the restoration of lost vision, through the application of the most sophisticated available techniques. Four resource and service Cores will help achieve the broad objective, as follows: I. Image Analysis and Graphics Core: Morphometric analysis; computer-aided image analysis; production of graphics for data analysis, presentation and publication II. Morphology Core: Histology (paraffin and plastic embedding, sectioning and staining, frozen sectioning for immunohistochemistry), ocular imaging (slit lamp examination and photography, Micron III fundus photography (with fluorescein angiography) and optical coherence tomography (OCT), biometric Bioptigen OCT) and visual functional testing (ERG, OptoMotry and IOP) and in vivo rodent eye injection with electroporator set up. Microscopy (light microscopy - brightfield, darkfield, phase contrast, DIC and fluorescence) and photomicrography, and advanced microscopy using a combination of spinning disk and confocal microscopes). III. Computer/IT Core: Programming for custom research needs; assistance in computer and information technology hardware and software selection, installation, instruction in use, maintenance and minor repairs. IV. Rapid-Prototyping and Design Core: Design of new unique research equipment plus education and training of users, empowering them to participate in the manufacturing, construction, maintenance, and upgrading of their unique research equipment designs. This is an application of a NEI Center Core Grant for Vision Research competing renewal submitted by the Principal Investigator and 19 other vision scientists who hold 23 active NEI R01 research grants. In addition, the UCSF vision research community supported by the NEI Vision Core Grant comprises 14 NEI- supported scientists with grant mechanisms other than active R01, 4 with other NIH funding, 1 with FDA R01 funding and 5 with private funding. There are 41 Core Investigators with active research programs, overall, each using at least one Core at a moderate or extensive level. Using traditional and innovative approaches, this Core Vision Research Grant has been highly successful and instrumental in enhancing the productivity and impact of vision research, attracting scientists to vision research and facilitating collaborative studies on the visual system at UCSF.