Shared Resource 06: Comparative Pathology and Digital Imaging (CPDISR)

项目摘要-比较病理学和数字成像共享资源(CPDISR) CPDISR为研究人员提供专业的、容易获得的、负担得起的实验病理学支持 利用临床前动物模型进行研究以了解癌症的发展和治疗 和/或为转译研究采购的人体组织。在本供资期间，最近的一项主要工作 开发包括为人体组织优化和扩展的完整组织学服务 为染色的人和动物组织载玻片提供数字化和定量图像分析服务。结果, Anil Parwani博士(MCC)与Krista La Perle博士(CB)被任命为联席主任，共享的名称 资源(SR)已更改为比较病理学和数字影像共享资源，并且 具体目标也作了相应的修改。具体目标是：1)提供咨询服务 OSUCCC研究人员，负责优化实验设计、样本提交和数据分析以及互动 与其他SR；2)为临床前疗效和毒性动物研究以及翻译提供支持 利用人体组织的研究；以及3)对染色的玻片进行数字化和定量图像分析 来自动物和人类组织。在目前的5年赠款周期内，第三委员会认证的兽医 已经增加了病理学家和七名额外的技术人员，以预期与 强劲的OSUCCC招聘和战略优先事项。CPDISR还购买了徕卡PELORIS 3双蒸锅 组织处理器在通过OSUCCC内部研究计划成功获得同行评审资金后。 在这一赠款周期内，CPDISR向84份出版物(12&>10个影响因素)、537名调查人员、 和57个NCI补助金，包括1个K12、1个K22、1个K24、5个P01、1个P50、35个R01、7个R21、2个R35、1个T32和3个U01 涉及所有五个OSUCCC研究计划的成员。在下一个赠款周期，CPDISR将是一个常规的 免疫监测和发现平台的成员，这是一个重要的共享资源倡议，促进 复杂的免疫肿瘤学项目。此外，一个主要的重点将是继续扩大我们的可用 针对组织中的免疫组织化学、免疫荧光和原位杂交优化的抗体菜单 动物和人类来源的各种染色平台，包括根据具体需求进行多路转换 OSUCCC成员的名单。我们还将增加对允许机器学习的图像分析项目的支持 将与癌症工程中心共同开发方法；并继续评估可用的开放 源和商业图像分析软件平台/模块，以补充我们的Aperio和Visiopamm 软件，以量化动物和人类组织中的生物标记物。CPDISR的年度预算为 1,863,254美元，但CCSG的请求是107,366美元。因此，CPDIR只寻求5.8%的预算支持 CCSG基金。

PROJECT SUMMARY – COMPARATIVE PATHOLOGY & DIGITAL IMAGING SHARED RESOURCE (CPDISR) The CPDISR provides expert, readily available and affordable experimental pathology support to investigators conducting research for understanding the development and treatment of cancer using preclinical animal models and/or human tissues procured for translational research. During the current funding period, a recent major development was the inclusion of complete histology services optimized for human tissues, as well as expanded slide digitization and quantitative image analysis services for stained human and animal tissue slides. As a result, Dr. Anil Parwani (MCC) has been named co-Director with Dr. Krista La Perle (CB), the name of the shared resource (SR) has been changed to the Comparative Pathology & Digital Imaging Shared Resource, and the Specific Aims have been modified accordingly. The Specific Aims are to: 1) provide consultative services to OSUCCC investigators for optimal experimental design, sample submission and data analysis, and interactions with other SRs; 2) provide support for preclinical efficacy and toxicity animal studies as well as translational studies that utilize human tissues; and 3) perform digitization and quantitative image analysis of stained slides from animal and human tissues. During the current 5-year grant cycle, a third board certified veterinary pathologist and seven additional technical staff have been added to anticipate usage in conjunction with the robust OSUCCC recruitment and strategic priorities. The CPDISR also acquired a Leica PELORIS 3 dual retort tissue processor after successful peer-reviewed funding through the OSUCCC Intramural Research Program. During this grant cycle, the CPDISR contributed to 84 publications (12 > 10 impact factor), 537 investigators, and 57 NCI grants, including 1 K12, 1 K22, 1 K24, 5 P01s, 1 P50, 35 R01s, 7 R21s, 2 R35s, 1 T32, and 3 U01s involving members of all five OSUCCC research programs. For the next grant cycle, the CPDISR will be a regular member of the Immune Monitoring and Discovery Platform, an overarching shared resource initiative fostering complex immuno-oncology projects. In addition, a major emphasis will be to continue to expand our available menu of antibodies optimized for immunohistochemistry, immunofluorescence and in situ hybridization in tissues of animal and human origin by various staining platforms, including multiplexing, according to the specific needs of OSUCCC members. We will also increase our support of image analysis projects allowing machine learning approaches to be developed with the center for cancer engineering; and continue to evaluate available open source and commercial image analysis software platforms/modules to complement our Aperio and Visiopharm software in order to quantify biomarkers in animal and human tissues. The annual budget of the CPDISR is $1,863,254, yet the CCSG request is $107,366. As such, the CPDIR seeks only 5.8% budgetary support from CCSG funds.