Center for Cellular Metabolism Research in Oklahoma

项目摘要/摘要 OMRF运营着三个小鼠屏障设施，其中唯一的无菌小鼠设施(GFM)和患者衍生的 俄克拉荷马州的异种移植核心(PDX)。所有五个研究项目负责人(RPL)对此的研究计划 新的Cobre使用老鼠模型，这些模型约占美国每日老鼠数量的15% OMRF鼠标设施。随着这些研究人员在未来五年扩大他们的研究项目，这 预计百分比将增加到~22%。此外，CMRO Cobre导师的实验室也 利用老鼠模型，导师和RPL加在一起目前几乎占人口普查的60%。 对老鼠笼子和辅助设备进行可靠的消毒对于老鼠屏障设施是必不可少的， 尤其是那些拥有免疫缺陷和无菌小鼠的小鼠。OMRF目前的灭菌器已有20年的历史， 失败了。为了解决这个问题，OMRF计划更换故障的消毒器，并在未来增加第二个消毒器 扩张。本附录要求对消毒设备提供部分支持。OMRF将提供50%的 这台设备的总成本。所要求的设备对于可重现的研究结果至关重要 CMRO Cobre RPL和他们的导师以及动物健康和福祉。此外，该设备 还将使俄克拉荷马州其他机构的其他符合科布雷和科布雷资格的调查人员受益 (俄克拉荷马大学健康科学中心和俄克拉荷马州立大学)，作为GFM和PDX 在该州的其他地方找不到设施。

PROJECT SUMMARY/ABSTRACT OMRF operates three mouse barrier facilities with the only germ-free mouse facility (GFM) and patient-derived xenograft core (PDX) in Oklahoma. The research programs of all five Research Project Leaders (RPLs) on this new COBRE use mouse models, which make up approximately 15% of the daily mouse population of the OMRF mouse facilities. As these investigators grow their research programs in the next five years, this percentage is expected to increase to ~22%. In addition, the laboratories of the CMRO COBRE mentors also utilize mouse models, and the mentors and RPLs combined currently constitute almost 60% of the census. Reliable sterilization of mouse caging and ancillary equipment is essential for mouse barrier facilities, especially those housing immunodeficient and germ-free mice. OMRF’s current sterilizer is 20 years old and is failing. To address this, OMRF plans to replace the failing sterilizer and add a second sterilizer for future expansion. This supplement requests partial support of the sterilizing equipment. OMRF will provide 50% of the total cost for this equipment. The requested equipment is critical for reproducible results for research of the CMRO COBRE RPLs and their mentors as well as animal health and well-being. Additionally, this equipment will also benefit other COBRE and COBRE-eligible investigators from additional institutions in Oklahoma (University of Oklahoma Health Sciences Center and Oklahoma State University), as the GFM and PDX facilities are not found elsewhere in the state.