Nevada Center of Biomedical Research Excellence in Molecular and Cellular Signal Transduction in the Cardiovascular System

我们第一阶段生物研究卓越中心(COBRE)的总体目标是 在分子和细胞信号转导方面建立内华达眼镜蛇 内华达大学雷诺分校(UNR)和北卡罗来纳大学医学院心血管系统 医学(UNR Med)作为可持续的、专题的、跨学科的基础科学研究 中心。这项任务将通过开发和利用最先进的分子， 遗传、生物物理和生物医学成像技术。眼镜蛇包括一个 管理核心(核心A)，由项目主任Scott Earley博士领导，两项研究 核心，以及5个项目负责人。玉梅·冯·厄利博士是转基因动物的负责人 基因分型和表型分析核心(核心B)和诺曼德·勒布朗博士领导高级空间 和时间分辨率成像核心(HSTRI；核心C)，它为项目负责人提供 能够访问各种最先进的仪器，从而实现高空间和时间 用荧光指示剂标记的细胞和组织样本的分辨率成像。眼镜蛇 正在提交行政补充申请，以回应NOSI NOT-GM-21- 029这将增强HSTRI核心的能力。这个应用程序的具体目的是 购买新的徕卡Stellaris SP5激光扫描共聚焦显微镜，以取代 老式奥林巴斯FV1000激光扫描共聚焦显微镜。这个新系统的价格是 316,286.70美元。如果NIGMS提供250,000美元，剩余的66,286.70美元将通过 制度保障。Cobre使用最多的显微镜FV1000系统 由HSTRI核心提供服务的项目负责人和其他UNR调查人员，购买于 2010年。不幸的是，显微镜最近变得不可靠了，120天中有51天 仅在2021年，停机时间就达到了42.5%，从而达到了其使用寿命的极限。这些 反复的失败对该中心的研究生产力产生了负面影响，这是一种情况 这将需要在未来找到一个更持久的解决方案。徕卡Stellaris SP5系统 与奥林巴斯FV1000相比，显著提高了时间和空间分辨率， 这是我们中心的几名研究人员的关键特征(所有5名科布雷项目负责人)， 包括该中心更成熟成员的研究计划，以及其他UNR 生物医学研究人员。按照配置，显微镜具有20 nm的自适应对焦控制 重新定位精度，这是FV1000所缺乏的，可以执行FRET、FRAP和Flip 实验，并可以很容易地升级到执行超分辨率显微镜。因此， 徕卡Stellaris SP5系统将取代和提升FV1000的功能。

The overall objective of our Phase I Center of Biological Research Excellence (COBRE) is to establish the Nevada COBRE in Molecular and Cellular Signal Transduction in the Cardiovascular System at the University of Nevada, Reno (UNR) and the UNR School of Medicine (UNR Med) as a sustainable, thematic, interdisciplinary basic science research Center. This mission will be accomplished by developing and utilizing state-of-the-art molecular, genetic, biophysical, and biomedical imaging technologies. The COBRE comprises an Administration Core (Core A) headed by the Program Director, Dr. Scott Earley, two Research Cores, and 5 Project Leaders. Dr. Yumei Feng Earley is the Director of the Transgenic Animal Genotyping and Phenotyping Core (Core B) and Dr. Normand Leblanc directs the High Spatial and Temporal Resolution Imaging Core (HSTRI; Core C), which provides the Project Leaders with access to a wide array of state-of-the-art instruments enabling high spatial and temporal resolution imaging of cells and tissues samples labeled with fluorescent indicators. The COBRE is submitting an application for an Administrative Supplement in response to NOSI NOT-GM-21- 029 that will enhance the capabilities of the HSTRI Core. The Specific Aim of this application is to purchase a new Leica STELLARIS SP5 Laser Scanning Confocal Microscope to replace an old Olympus FV1000 Laser Scanning Confocal Microscope. The price tag for this new system is $316,286.70. If NIGMS provides $250,000, the remaining $66,286.70 will be provided through institutional support. The FV1000 system, which is the most utilized microscope by COBRE Project Leaders and other UNR investigators served by the HSTRI Core, was purchased in 2010. Unfortunately, the microscope has recently become unreliable with 51 out of 120 days of down time (42.5%) in 2021 alone and has thus reached the limit of its usable life. These repetitive failures have negatively impacted the research productivity of the Center, a situation that will require a more permanent solution in the future. The Leica STELLARIS SP5 system dramatically increases the temporal and spatial resolution relative to the Olympus FV1000, which are key features for several investigators in our Center (all 5 COBRE Project Leaders), including the research programs of more established members of the Center, and other UNR biomedical investigators. As configured, the microscope has Adaptive Focus Control with 20 nm repositioning accuracy, which the FV1000 lacks, and can perform FRET, FRAP, and FLIP experiments, and could be easily upgraded to perform super-resolution microscopy. Thus, the Leica STELLARIS SP5 system will replace and advance the capabilities of the FV1000.