Social Stress, Epigenetics and Cardiometabolic Health Among Youth

项目摘要 有大量证据表明，经济和社会压力塑造心血管疾病的发展 疾病在生命早期就开始了。然而，在很大程度上不知道社会和经济压力是如何产生的。 影响生物过程，特别是在细胞水平上，以及这些过程是否明显 在儿童和青少年中。拉丁美洲人是世界上最大和增长最快的少数民族群体。 在美国，心血管风险因素的发病率很高，贫困率也较高 与其他种族/民族群体相比，儿童期和成年期的社会压力源。一个新颖 方法来评估潜在的机制之间的关联压力和 在整个生命过程中影响心血管健康的关键因素是DNA甲基化。最近，一些小型研究 发现压力经历可能会改变DNA甲基化，从而提供了一种潜在的机制， 哪些社会压力源可能会刺激皮肤。我们建议研究社会与经济之间的联系， 压力源，与DNA甲基化，线粒体DNA（mtDNA）损伤和心脏代谢健康， 参加西班牙裔社区健康研究/拉丁裔研究（HCHS/SOL）的儿童中 Study. HCHS/SOL青年研究是对美国拉丁美洲人的研究，代表不同的原籍国， 在美国进行。我们建议使用完成HCH/SOL的1200名参与者的现有数据 以及谁提供了血样。将对现有血液样本进行DNA甲基化分析 年龄、全基因组甲基化和mtDNA损伤标记。心脏代谢健康标志物（肥胖， 糖尿病、高血压、炎症和脂质）。现有数据还包括 评估儿童目前的家庭环境以及社会文化因素。具体地说， 我们将研究1）社会和经济压力是否与DNA甲基化年龄相关， mtDNA损伤和全基因组甲基化; 2）DNA甲基化是否随年龄、全基因组 甲基化和线粒体DNA损伤与儿童的心脏代谢健康有关; 3） 社会文化因素（即种族认同、父母关系、出生地）改变了 社会和经济压力以及DNA甲基化和mtDNA损伤关联。我们将进一步 实施孟德尔随机化技术，以解决数据的横截面性质。 这个项目的完成将使我们能够阐明社会和经济压力因素对 表观遗传和心脏代谢标志物可能有助于解释压力如何塑造持久的人口 拉丁美洲人口的健康差距。

Project Summary There is extensive evidence that economic and social stressors shape the development of cardiovascular disease starting early in the life course. However, it is largely unknown how social and economic stressors affect biological processes, particularly at the cellular level and whether these processes are evident among children and adolescents. Latinos, the largest and fastest growing ethnic minority group in the United States, have a high prevalence of cardiovascular risk factors and experience higher rates of poverty and social stressors in both childhood and adulthood compared to other racial/ethnic groups. One novel method to evaluate possible mechanisms underlying the associations between stressors and cardiovascular health across the life course is DNA methylation. Recently a handful of small studies have found that stressful experiences may alter DNA methylation, thus providing a potential mechanism by which social stressors may get under the skin. We propose to examine the association between social stressors, in relation to DNA methylation, mitochondrial DNA (mtDNA) damage and cardiometabolic health, among children participating in the Hispanic Community Health Study /Study of Latinos (HCHS/SOL) Youth Study. The HCHS/SOL Youth study is a study of US Latinos, representing varied countries of origin, conducted in the US. We propose to use existing data from 1200 participants who completed the HCH/SOL Youth study and who provided blood samples. Existing blood samples will be assayed for DNA methylation age, genome-wide methylation and a mtDNA damage marker. Cardiometabolic health markers (obesity, diabetes, hypertension, inflammation and lipids) have also been assessed. Existing data also includes assessments of current family environment as well as socio-cultural factors among children. Specifically, we will examine 1) whether social and economic stressors are associated with DNA methylation age, mtDNA damage and genome-wide methylation; 2) whether DNA methylation age, genome-wide methylation and mtDNA damage is associated with cardiometabolic health among children and 3) whether socio-cultural factors (i.e, ethnic identity, parental closeness, place of birth) modify the association between social and economic stress and DNA methylation and mtDNA damage association. We will furthermore implement mendelian randomization techniques to address the cross-sectional nature of the data. Completion of this project would allow us to elucidate the impact social and economic stressors have on epigenetic and cardiometabolic markers that may help explain how stress shapes persistent population health disparities among Latino populations.