Flow Cytometery and Cell Sorting Core

核心B-Flow细胞术和细胞分选核心(核心领导：Porcelli)计划领导：Backer，Jonathan M. 摘要 流式细胞术分析和细胞分选核心(核心B)将提供设备和专门知识 用于各种先进的基于流式细胞术的单细胞水平分析和分离技术。这个 CORE将支持和维护一系列用于分析和分类应用的先进仪器，并将 积极指导项目研究人员将这些应用于癌症转移的研究。这些 将应用方法学来识别、分离和详细描述感兴趣的细胞类型和 与计划项目的目标相关。核心资源将在所有项目中广泛使用 组成本计划，他们在此结构下的组织和支持将为本计划提供 以具有成本效益的方式为调查人员提供访问和技术支持。核心关键人员将 致力于直接协助项目绩效指标制定和实施单项 与他们的项目相关的细胞分析技术，并帮助他们使用 核心技术。通过为基于流式细胞术的单细胞组合提供组织核 分析和隔离方法，核心B将创建基础架构和环境以适应这些 不断发展的技术，以满足PPG调查人员的需求。所有必要的设备和专业知识 这一核心所需的信息已经到位，并可在我们的机构获得。关键人员与必要的 已经从现有教职员工中招聘了背景和专业知识，这些人已经 被指定为适当的努力水平，以确保核心B的正常运作。 与核心相关的设备和人员在我们的 NCI CCSG(P30)资助的流式细胞仪核心设备，核心B的创建是成功的关键 实现PPG的目标，因为它将使这些技术能够在新的 和创新的应用，专门解决形成主题的癌症转移的研究 当前程序应用程序。 PHS 398/2590(11/07版)页面

Core B – Flow Cytometry and Cell Sorting Core (Core Lead: Porcelli) Program Lead: Backer, Jonathan M. ABSTRACT The Flow Cytometry Analysis and Cell Sorting Core (Core B) will provide access to equipment and expertise for a variety of advanced flow cytometry-based technologies for single cell level analysis and isolation. The core will support and maintain a range of advanced instruments for analytic and sorting applications, and will actively guide the program investigators in applying these to their studies on cancer metastasis. These methodologies will be applied for the identification, isolation and detailed profiling of cell types of interest and relevance to the goals of the program project. The resources of the core will be used extensively in all projects comprising this program, and their organization and support under this structure will provide the Program investigators with access and technical support in a cost effective way. The key personnel of the core will have effort dedicated to directly assisting the program PIs in the development and implementation of single cell analysis techniques relevant to their projects, and assist them in innovating new approaches using the core technologies. By providing an organizing nucleus for a portfolio of flow cytometry-based single cell analysis and isolation methods, Core B will create an infrastructure and environment for adapting these evolving technologies to the needs of the PPG investigators. All of the essential equipment and expertise required for this core are already in place and available at our institution. Key personnel with the necessary background and expertise have been recruited from existing faculty and staff, and these individuals have been designated appropriate levels of effort to ensure proper functioning of Core B. While some of the equipment and personnel associated with the core are physically housed and partially supported within our NCI CCSG (P30) funded Flow Cytometry Core Facility, the creation of Core B is essential to the successful accomplishment of the goals of the PPG, as it will enable the implementation of these technologies for new and innovative applications that specifically address the studies of cancer metastasis that form the theme of the current program application. PHS 398/2590 (Rev. 11/07) Page