Cell fate choices by Tbx1 in forming the mammalian heart

Tbx1编码心脏发育所需的T-box转录因子。这个基因映射到 DiGeorge综合征/心动过速患者染色体22q11.2区缺失 面部综合征或22q11.2缺失综合征(22q11.2DS)。约60%的22q11.2DS 患者患有先天性心脏病，主要影响心脏流出道。的子集 已经鉴定出具有TBX1基因突变但没有缺失的个体，他们 部分表型患者有22q11.2DS。小鼠中TBX1的一个等位基因失活导致 轻度缺陷，但两个等位基因都失活会导致持续性干新生儿死亡 动脉硬化症，其中的主动脉和肺动脉干不能分离。这一缺陷也发生在5- 22q11.2DS合并先天性心脏病的占10%。要了解TBX1在 ，我们进行了心咽单细胞rna测序(scrna-seq)。 咽器官内的中胚层祖细胞。我们发现了一个多血统 表达对形成心脏流出道重要的基因的祖细胞(MLP)群体 以及面部和颈部的臂肌。MLP的人口在 当TBX1在心咽部失活时，更多分化的群体的代价 中胚层。我们的主要假设是Tbx1在MLP人群中是疾病进展所必需的 心脏发育所需的更多分化状态。我们提出了三个具体的 旨在检验这一假说。在第一个目标中，我们将进行额外的scrna-seq实验。 并分析完整的数据集，以了解MLP细胞的进展是如何在 TBX1条件突变和全局突变小鼠胚胎。在目标2中，我们将确定MLP的位置 细胞定位于胚胎中。初步数据显示，这些细胞定位于 伸长的咽器的新生间充质。我们还将停用TBX1 特别是在MLP细胞内，以确定其特定功能。在目标3中，我们将转向 功能基因组研究，并将确定开放的和可获得的染色质 利用ATAC-SEQ和CHIP-SEQ从胚胎组织中获得了Tbx1蛋白结合位点。初步数据 这表明我们能够识别直接转录的靶基因。通过这三个目标，我们 将了解TBX1在祖细胞向更多 分化状态以建立心脏流出道。

TBX1 encodes a T-box transcription factor required for cardiac development. This gene maps to the chromosome 22q11.2 region that is deleted in patients with DiGeorge syndrome/velo-cardio- facial syndrome or 22q11.2 deletion syndrome (22q11.2DS). Approximately 60% of 22q11.2DS patients have congenital heart disease that mostly affects the cardiac outflow tract. A subset of individuals with a mutation of the TBX1 gene but not a deletion has been identified and they partially phenocopy patients with 22q11.2DS. Inactivation of one allele of Tbx1 in mice results in mild defects, but inactivation of both alleles results in neonatal lethality with a persistent truncus arteriosus, in which the aorta and pulmonary trunk fail to separate. This defect also occurs in 5- 10% of 22q11.2DS patients with congenital heart disease. To understand the function of Tbx1 in mammals, we performed single cell RNA-sequencing (scRNA-seq) of cardiopharyngeal mesoderm progenitor cells within the pharyngeal apparatus. We discovered a multilineage progenitor (MLP) population that expresses genes important for forming the cardiac outflow tract as well as branchiomeric muscles of the face and neck. The MLP population expands at the expense of more differentiated populations when Tbx1 is inactivated in the cardiopharyngeal mesoderm. Our main hypothesis is that Tbx1 is required in the MLP population for progression towards more differentiated states needed for cardiac development. We propose three specific aims to test this hypothesis. In the first aim, we will perform additional scRNA-seq experiments and analyze the complete dataset to understand how the progression of MLP cells are altered in Tbx1 conditional and global mutant mouse embryos. In Aim 2, we will determine where the MLP cells are localized in the embryo. Preliminary data suggests that these cells are localized to the nascent mesenchyme of the elongating pharyngeal apparatus. We will also inactivate Tbx1 specifically within the MLP cells to determine its particular functions. In Aim 3, we will turn to functional genomic studies and will identify open and accessible chromatin for which harbors TBX1 protein binding sites using ATAC-seq and ChIP-seq from embryo tissue. Preliminary data suggests that we are able to identify direct transcriptional target genes. By these three aims, we will understand the molecular functions of TBX1 in the progression of progenitor cells to more differentiated states to build the cardiac outflow tract.