Extending NARMS surveillance of antibiotic resistance in retail foods in California

项目总结/摘要 抗生素耐药性（AMR）是食品安全和公共卫生的重要问题。的 国家抗菌素耐药性监测系统（NARMS）零售食品监测 南加州于2018年启动。该项目将继续监测在最 人口稠密的城市洛杉矶，并扩大监测到农村地区的加州。的 该项目的目标是提高食品安全和保护公众健康， 开展以下具体目标和活动。首先，我们将继续NARMS零售 洛杉矶市的食品监测，并将监测范围扩大到两个农村地区 （阿马多尔和卡拉维拉斯）在加州。其次，我们将监测不同地区的AMR趋势， 零售肉类和海鲜样本中的病原体。我们将收集零售肉类（鸡肉，火鸡， 猪肉、牛肉）和海鲜（虾、鲑鱼、罗非鱼）样本，每月从随机抽取 杂货店我们将测试肉类样本的沙门氏菌，弯曲杆菌（仅家禽），大肠杆菌。杆菌 以及肠球菌和海产品样品中的肠球菌、气单胞菌、弧菌等乳糖 根据NARMS的零售食品协议。细菌分离株将被送往 FDA每月进行抗生素敏感性测试（AST）和全基因组测序（WGS） (WGS将由UC Davis完成）。第三，我们将进行流行病学调查， 研究零售食品中抗生素耐药性的流行情况。我们将收集商店信息和元数据， 每个零售肉类和海鲜样本，并确定流行之间的关键联系， 细菌及其AMR（如单一或多重耐药性）和种属、来源、类型，以及 零售食品的形式，抗生素使用的声明，以及季节等。第四，根据本研究的结果， 项目，我们将制定奠定出版物，宣传材料，摘要和海报，和同行， 审查出版物。我们将向农业和水产养殖界传播研究结果， 兽医和公众通过通讯，推广讲习班，在会议上的介绍， 会议和同行评审期刊。第五，我们将参加NARMS电话会议 和工作组。 通过实现这些具体目标和开展活动，该项目将反映以下趋势： 加州州零售食品中的AMR。该项目将加强NARMS零售食品 监测和提高公众对零售食品中抗生素耐药性的认识。该项目还将 加强联邦和州机构与学术界在研究、检测 监测和调查与零售食品中抗生素耐药性相关的食源性疫情。 1

Project Summary/Abstract Antimicrobial resistance (AMR) is an important issue for food safety and public health. The National Antimicrobial Resistance Monitoring System (NARMS) Retail Food Surveillance in southern California started in 2018. This project will continue the surveillance in the most populated city of Los Angeles and expand the surveillance to rural areas in California. The project’s goal of improving food safety and protecting public health will be achieved by undertaking the following specific aims and activities. First, we will continue the NARMS retail food surveillance in the city of Los Angeles and expand the surveillance to two rural areas (Amador and Calaveras) in California. Second, we will monitor trends of AMR in different pathogens in retail meat and seafood specimens. We will collect retail meat (chicken, turkey, pork, beef) and seafood (shrimp, salmon, tilapia) specimens monthly from randomly selected grocery stores. We will test meat samples for Salmonella, Campylobacter (poultry only), E. coli and Enterococcus and seafood samples for Enterococcus, Aeromonas, Vibrio and other lactose positive bacteria according to NARMS’ Retail Food Protocols. Bacterial isolates will be sent to FDA monthly for antimicrobial susceptibility test (AST) and whole genome sequencing (WGS) (WGS of meat isolates will be done by UC Davis). Third, we will conduct epidemiological studies on prevalence of AMR in retail food. We will collect store information and metadata for each retail meat and seafood specimen and determine key links between the prevalence of bacteria and their AMR (e.g. single or multiple drug resistance) and species, origins, types, and forms of retail food, claims of antibiotics use, and seasons etc. Fourth, based on results of this project, we will develop lay publications, outreach materials, abstracts and posters, and peer- reviewed publications. We will disseminate findings to agriculture and aquaculture communities, veterinarians, and the public via newsletters, extension workshops, presentations in meetings and conferences, and peer-reviewed journals. Fifth, we will participate in NARMS conference calls and working groups. By accomplishing these specific aims and activities, the project will characterize the trends of AMR in retail foods in the state of California. The project will enhance NARMS Retail Food Surveillance and increase public awareness of AMR in retail foods. The project will also strengthen collaborations among federal and state agencies and academia on research, detection, surveillance, and investigation of foodborne outbreaks associated with AMR in retail food. 1