Tracking DNA repair dynamics in the nuclear landscape of a living cell

本项目旨在追踪活细胞核景观中DNA修复因子的招募，并量化核结构在维持基因组完整性中的作用。通过将先进的荧光显微镜与一种新型DNA双链断裂诱导细胞系统相结合，该项目有望揭示细胞核如何在空间上实时协调DNA损伤检测、评估和修复。这项研究很重要，因为DNA损伤威胁着生物体的生存，而这个项目有可能定义这种基因组威胁如何在单分子水平上解决。这项研究的好处是对DNA修复生物学的基本见解和成像技术的发展，以量化基因组功能。

This project aims to track DNA repair factor recruitment in the nuclear landscape of a living cell and quantify the role of nucleus architecture in maintenance of genome integrity. By coupling advanced fluorescence microscopy with a novel DNA double strand break inducible cell system, this project expects to uncover how the nucleus spatially coordinates DNA damage detection, assessment and repair in real time. This research is important because DNA damage threatens organism survival and this project has the potential to define how this genomic threat is resolved at the single molecule level. The benefit of this research is a fundamental insight into DNA repair biology and development of imaging technology to quantify genome function.