Tissue Core-UNMC Rapid Autopsy Program

项目摘要摘要 此Artnet应用程序中的所有项目都将使用已识别的人类样本进行研究 了解胰腺导管腺癌患者对治疗的获得性抵抗。为了提供 为了获得必要的标本，已与联合国儿童基金会合作并在其赞助下开发了组织核心。 内布拉斯加大学医学中心(UNMC)组织银行，目标是提供协调和 集中的核心，专门用于采购、处理和分发生物标本以及与之相关的数据 确定了临床数据。快速尸检程序(RAP)核心设施存储正常、良性(即急性 和/或慢性纤维性胰腺炎)和恶性胰腺组织(包括原发和转移性 胰腺癌以及神经内分泌肿瘤)和外周血淋巴细胞、血浆和 胰腺癌手术患者和已故患者的血清 有胰腺癌病史的患者(快速尸检)。非癌症捐赠者整个胰腺，脾， 十二指肠、肝、肺、肌肉、淋巴结和肾上腺组织也与当地合作收集 器官采购机构。RAP生物库还与外科和肿瘤学以及 几个建立良好的UNMC患者监测计划，如PCDC和PCCR，以制定 稳健的前期、早期和末期组织和生物体液样本集的纵向收集。重要的是， 目前可从快速尸检方案获得的存档样本是基于UNMC的资源 代表了研究PDAC疾病获得性耐药性所需的确切样本集 这一资源的持续增长只会有助于提高军警部队研究抗病能力的能力。这个 CORE还维护着可供所有癌症研究人员使用的各种资源的缓存，如疾病和 正常TMA、供体样本序列分析和新鲜组织采集。该银行包括一个 数据库管理和标本分发机制(Freezerworks)和统一的 组织银行监督委员会已经确定并使用了所需材料的优先顺序。 这一核心设施旨在使ARTnet的具体研究活动以及研究 UNMC内外专注于翻译研究的其他科学家的活动 问题和阻力机制。 只有在所有其他诊断程序后从临床适应症手术中获得的标本才有 将被提交给UNMC Artnet组织库进行耐药性研究。这个 否则，标本将被丢弃或处置。符合条件的患者有机会参加 提交书面知情同意书。不会对患者造成风险，也不会对患者的护理造成损害， 因为执行的所有程序都是为了诊断而执行的，而不考虑收集需要。 。

Project Summary Abstract All projects in this ARTNet application will use deidentified human specimens for research directed at understanding acquired resistance to therapy in pancreatic ductal adenocarcinoma. In order to provide the necessary specimens, a Tissue Core has been developed in cooperation with and under the auspices of the University of Nebraska Medical Center (UNMC) Tissue Bank, with the goal of providing a coordinated and centralized core dedicated to procuring, processing and distributing biological specimens and associated de- identified clinical data. The Rapid Autopsy Program (RAP) core facility stores normal, benign (i.e. acute and/or chronic fibrosing pancreatitis) and malignant pancreatic tissues (including both primary and metastatic pancreatic carcinomas as well as neuroendocrine tumors) and peripheral blood lymphocytes, plasma and serum from patients undergoing surgical procedures for pancreatic malignancies and from deceased patients with a history of pancreatic cancer (rapid autopsy). Non-cancer donor whole pancreata, spleen, duodenum, liver, lung, muscle, lymph node and adrenal tissue are also collected in collaboration with local organ procurement agencies. The RAP biorepository also coordinates with surgery and oncology, as well as several well-established UNMC patient monitoring programs, such as the PCDC and PCCR, to develop robust longitudinal collections of pre, early, and end-stage tissue and biofluid sample sets. Importantly, the currently archived samples available from the rapid autopsy program are a UNMC-based resource representing the exact sample sets necessary to investigate acquired resistance in PDAC disease and continued growth of the resource will only serve to enhance UNMC’s ability to study disease resistance. The core also maintains a cache of various resources available to all cancer investigators, such as disease and normal TMAs, donor sample sequence analysis and fresh tissue acquisition. The bank includes a mechanism for database management and specimen distribution (Freezerworks) and a uniform system of prioritization of requested materials has been defined and used by the Tissue Bank Oversight Committee. This core facility is intended to benefit the specific research activities of the ARTNet, as well as the research activities of other scientists within and outside of UNMC who are concentrating on translational research issues and resistance mechanisms. Only specimens obtained from clinically indicated surgeries after all other diagnostic procedures have been performed will be submitted to the UNMC ARTNet tissue bank for resistance research. The specimens would otherwise be discarded or disposed. Eligible patients have the opportunity to participate by submitting written informed consent. There is no risk to the patient or compromise to the patient's care, as all the procedures performed would be performed for diagnostics regardless of the collection need. .