Core B-Omics and Gene-Editing

核心B-摘要 分子健康和疾病中心(MCHD)的目标是促进中心主题下的研究 以增强分子生理学的教育深度，指导和培训应用研究人员 跨健康-疾病连续体的组学技术和计算生物学。对遗传学的理解 涉及疾病发生和发展的易感性和分子机制有可能导致 早期干预以及新的治疗目标。基因组、转录组、蛋白质组、 使用计算方法的新陈代谢数据集以及生理信息可以提供 对与健康-疾病连续体相关的分子、细胞和整体生理学的新见解。 MCHD的使命将通过多个组成部分的协同互动来实现，包括 核心A(包括行政监督、教育和指导方案以及一个试点项目方案)，两个 研究核心和三个主要项目调查人员，以解决健康和疾病的各种问题，使用 分子和计算方法。特别是，Core B-Omics和基因编辑将提供 研究人员获得了一条全面的最先进的组学数据收集管道，机制基因- 编辑和生物学方法，并与研究计算、生物信息学和 生物统计核心(核心C)将提供更深入和洞察与主要 项目和试点项目研究人员提出科学问题。核心B将加强目前的基础设施，包括 通过实施新的高通量测序方法，如空间转录和新设备 用于长读测序和结构变化以及发展新的创新能力 利用CRISPR/Cas9基因编辑技术询问基因功能和生物学途径。这个 核心B的目标是：(1)促进对教职员工、受训人员和学生的组学教育和培训 技术和基因编辑技术；(2)向MCHD研究人员提供技术专长和 尖端组学服务，包括下一代测序方法和质谱学 靶向蛋白质组学和代谢组学；(3)为MCHD研究人员提供先进的基因编辑和 研究人员发起科学研究病毒载体以研究基因功能和相关途径 问题；以及(4)寻求对现有分析、服务的持续改进，并加强技术 通过收购新的尖端仪器来提高能力，这将有助于 核心。总而言之，这些目标将与初级研究人员协同工作，以提供创新的、最先进的 基础设施，以增强和加强对健康和疾病的分子基础的调查， 支持校外研究资金的成功，并共同发展MCHD。

Core B-Abstract The goal of the Molecular Center of Health and Disease (MCHD) is to facilitate research under a central theme of molecular physiology to enhance the depth of education, mentorship, and training of researchers to apply omics technology and computational biology across the health-disease continuum. An understanding of genetic susceptibility and molecular mechanisms involved in onset and progression of disease has the potential to lead to early interventions as well as novel therapeutic targets. The integration of genomic, transcriptomic, proteomic, and metabolomic datasets along with physiological information using computational approaches can provide novel insight into the molecular, cellular, and overall physiology associated with the health-disease continuum. The mission of the MCHD will be achieved through synergistic interaction across multiple components, including Core A (including administrative oversight, education and mentoring programs, and a pilot project program), two research cores, and three major project investigators, to address diverse questions of health and disease using molecular and computational approaches. In particular, Core B- Omics and Gene-Editing will provide researchers access to a comprehensive state-of-the-art omics data collection pipelines, mechanistic gene- editing and biological approaches, and seamless integration with the Research Computing, Bioinformatics, and Biostatistics Core (Core C) will provide greater depth and insight into biological processes associated with major project and pilot program researcher scientific questions. Core B will enhance current infrastructure, including by implementing new high-throughput sequencing methods, such as spatial transcriptomics and new equipment for long-read sequencing and structural variation as well as the development of a new and innovative capability involving CRISPR/Cas9 gene-editing technology to interrogate gene function and biological pathways. The objectives of Core B are: (1) promote education and training of faculty, trainees, and students in omics technologies and gene-editing technologies; (2) provide MCHD investigators access to technical expertise and cutting-edge omics services, including next generation sequencing methodologies and mass spectrometry targeted proteomics and metabolomics; (3) provide MCHD investigators access to advanced gene-editing and viral vector development to investigate gene function and pathways associated with researcher initiated scientific questions; and (4) seek continuous improvement in existing assays, services, and enhance technological capabilities through acquisition of new cutting-edge instrumentation that will assist in the sustainability of the Core. In total, these aims will work in synergy with junior researchers to provide an innovative, state-of-the-art infrastructure to enhance and strengthen investigation into the molecular underpinnings of health and disease, bolster success in extramural research funding, and collaboratively grow the MCHD.