FLUORESCENT MICROSPHERE COUNTING FOR FLOW MEASUREMENTS

本研究的长期目标是开发低成本、准确的 用于组织中的局部流动测量的方法。本研究立足 关于组织中的荧光微球 可在荧光流动系统中准确、快速计数。的 该项目将开发仪器和评估程序， 微球在组织培养中的作用。在第一阶段， 荧光微球计数系统将组装和条件 用于测定微球的准确计数。的时间曲线 将评价微球通过情况，并将结果与 荧光分光光度法I期研究将证明 工作假设的可行性，并提供设计参数 在H阶段建造的自动化系统， 生理学研究环境，以评估其实用性和易用性。 该项目将为研究人员提供一个有价值的工具， 成本和劳动力参与区域流量测量，并提高 用荧光微球代替旧的 放射性微球法 建议的商业应用：该技术将取代目前的 用于估计具有潜在更多的微球的微球数目的方法 准确的方法，具有较低的资本，用品和劳动力成本。 潜在的市场包括目前使用的数百个实验室， 荧光微球和大量额外的研究人员 利用可供选择的放射性和有色微球方法。

The long term goal of this research is to develop low cost and accurate methods for regional flow measurements in tissues. This research is based on the working hypothesis that fluorescent microspheres in tissue digests can be accurately and rapidly counted in a fluorescence flow system. The project will develop instrumentation and evaluate procedures to count microspheres in tissue digests. During Phase I a continuous flow fluorescent microsphere counting system will be assembled and conditions for the accurate counting of microspheres determined. Time profiles of microsphere passage will be evaluated and results compared with the fluorescence spectrophotometry method. Phase I studies will demonstrate the feasibility of the working hypothesis and provide design parameters for automated systems to be constructed during Phase H for testing in physiology research environments to evaluate its utility and ease of use. This project will provide researchers with a valuable tool for lowering the cost and labor involved in regional flow measurements and improve the attraction of using fluorescent microspheres to replace the older radioactive microsphere methods. PROPOSED COMMERCIAL APPLICATION: This technology would replace current methods for estimating numbers of microspheres with a potentially more accurate method that has lower capital, supplies, and labor costs. Potential markets include the hundreds of laboratories currently using fluorescent microspheres and the large number of additional researchers utilizing the alternative radioactive and colored microsphere methods.