PRIMARY TOOTH DENTIN AS A MARKER FOR FLUORINE EXPOSURE

NIH最近主办的两次研讨会的记录表明， 对系统性氟暴露模式变化的关注， 氟中毒患病率和严重程度的增加。会议商定 有一个重要的需要，以监测接触的变化， 改进和更实用的方法来衡量累积系统 曝光是迫切需要的。 本申请书所述的项目 将决定收集的牙本质中的F-浓度（[F-]） 从脱落的乳牙（EPT）可以作为一个准确和有用的 监测婴儿期累积全身氟暴露的标志物， 童年. 具体目的是：（1）以大鼠磨牙为模型系统， 确定牙本质中氟吸收在 初级牙本质沉积和初级沉积完成后。 (2)为了从EPT中确定牙本质中的[F-]分布， 牙本质-牙釉质交界处的牙髓表面使用西姆斯，并涉及 将这些数据与来自各自氟化物暴露历史的信息进行比较， (FEH)龋病指数和氟中毒指数评分均高于对照组（P <0.05）。(3)探讨 牙本质中[F-]之间的关系（通过化学分析获得） 根据EPT估算的相应全身F-暴露量， 氟暴露史，氟中毒指数得分从 恒牙和龋齿指数得分。实地的用处- 将评估该方法的收集适应性。 具体目标#1和#2产生的结果将提供 生物学参数的重要信息的解释 从临床流行病学研究中获得的结果描述 具体目标#3我们假设这个项目的结果将 表明EPT测定牙本质中[F-]可作为一种准确、有效的 应用标记物监测婴儿期累积全身氟暴露 还有童年

The proceedings of two recent NIH-sponsored workshops have expressed concern over changing patterns of systemic fluoride exposure, as evidenced by increases in the prevalence and severity of fluorosis. It was agreed that there is an important need to monitor shifts in exposure, and that improved and more practical methods of measuring cumulative systemic exposure are urgently needed. The project described in this application will determine to what extent F- concentrations ([F-]) in dentin collected from exfoliated primary teeth (EPT) can serve as an accurate and useful marker to monitor cumulative systemic F- exposure during infancy and childhood. The Specific Aims are: (l) Using the rat molar as a model system, to define the relationship between F- uptake in dentin during the period of primary dentin deposition and after the completion of primary deposition. (2) To determine the [F-] profile in dentin from EPT, proceeding from the dentin-enamel junction to the pulpal surface using SIMS, and to relate these data to information from respective fluoride exposure histories (FEH), and to the caries and fluorosis index scores. (3) To investigate the relationships between [F-] in dentin (obtained by chemical analysis) from EPT, and the respective systemic F- exposure as estimated from fluoride exposure histories, fluorosis index scores taken from the permanent teeth, and caries index scores. The usefulness of the field- collection adaptations of the method will be evaluated. The findings generated from Specific Aims #1 and #2 will provide information on biologic parameters important to interpretation of the findings to be obtained from the clinical-epidemiological study described in Specific Aim #3. We hypothesize that the results of this project will show that [F-] in dentin from EPT can be used as an accurate and usefully applied marker to monitor cumulative systemic F- exposure during infancy and childhood.