Genome engineering and synthetic biology approaches for improving industrial CHO cell production of biologics

该项目旨在开发改进CHO细胞重组蛋白表达和生物生产平台的原则证明方法，其基础是：(I)合理的定点靶向基因组工程(Ii)基因电路设计，采用遗传反馈调控和条件调控的元素。通过这种方式，“难以表达”的蛋白质将对照基准黄金标准表达蛋白质进行测试，目的是以一种自我调节、广泛适用的方式提高蛋白质产量。

This project will aim to develop proof-of-principle approaches for improving CHO cell platforms for recombinant protein expression and bioproduction, based on combining: (i) rational site-targeted genome engineering (ii) gene circuit design, employing elements of genetic feedback regulation and conditional regulation. In this way, "difficult-to-express" proteins will be tested against a benchmark gold-standard expression protein, with the aim of improving protein production in a self-regulating, widely-applicable manner.