EFFECTIVENESS OF HTLV-III ANTIBODY SCREENING

本项目的目标是开发一种流式细胞仪检测系统。 (FCDS)用于感染HIV-1的人淋巴细胞。最优条件 已经建立了固定；这些不仅描绘了 细胞内艾滋病毒的存在，但也会导致灭活 传染性艾滋病病毒。此外，一次和二次抗体反应 也被刻画出来了。一抗是p24的特异性抗体， 二抗由a的f(ab‘)2片段组成 荧光素偶联，羊抗鼠免疫球蛋白抗体。这个 系统足够灵敏，每10,000个细胞中就有一个感染艾滋病毒的细胞 实验感染的人T淋巴细胞系中的未感染细胞。 FCDS目前正被用来检查已知的 感染艾滋病毒的风险很高(血友病患者和他们的性行为 合作伙伴)和随机挑选的献血者。该系统还具有 已被修改以包括使用Leu M3标记的藻红蛋白 用于识别受感染单核细胞的单抗(CD14)。低 在Leu M3中检测到明显阳性细胞的水平 阴性细胞。目前正在进行实验，以确定 不管这些细胞是否感染了艾滋病毒。

The goal of this project is to develop a flow cytometry detection system (FCDS) for human lymphocytes infected with HIV-1. Optimal conditions for fixation have been established; these not only delineate the presence of intracellular HIV, but also result in inactivation of infectious HIV. Furthermore, primary and secondary antibody reactions have also been characterized. The primary antibody is specific for p24, and the secondary antibody consists of the f(ab')2 fragment of a fluorescein-conjugated, goat anti-mouse immunoglobulin antibody. The system is sensitive enough to detect one HIV-infected cell in 10,000 uninfected cells in experimentally infected human T-lymphoid cell lines. The FCDS is currently being used to examine samples from people known to be at high risk of HIV infection (hemophiliacs and their sexual partners) and from randomly selected blood donors. The system has also been modified to include the use of leu M3 phycoerythrin-labelled monoclonal antibody (CD14) for identifying infected monocytes. Low levels of apparently positive cells have been detected in the leu M3 negative cells. Experiments are currently under way to identify whether or not these cells are infected with HIV.