IMMUNODOMINANT TARGETS OF CMI TO CRYPTOSPORIDIUM

描述：(改编自申请人摘要)。这一秒 重新提交题为“CMI对隐孢子虫的免疫优势靶点” 识别免疫反应中重要的隐孢子虫抗原和 恢复。申请人建议鉴定和分离重组C. 利用多克隆和单克隆技术从cDNA文库中提取细小病毒抗原 针对隐孢子虫蛋白产生的抗体，以评估 用T细胞纯化重组蛋白的免疫原性 以凝胶纯化蛋白或硝酸纤维素作增殖试验- 结合蛋白(T细胞免疫印迹法)以确定 这些抗原激发T细胞反应，最终分离T细胞 对这些抗原产生强烈反应的克隆，然后移植到 以确定这些药物是否可以减少寄生虫 使恢复或诱导恢复。

DESCRIPTION: (Adapted From Applicant's Abstract). This second resubmission entitled "Immunodominant Targets of CMI to Cryptosporidium" is to identify important cryptosporidial antigens in immune response and recovery. The applicant proposes to identify and isolate recombinant C. parvum antigens from a cDNA library using polyclonal and monoclonal antibodies generated against cryptosporidial proteins, to evaluate purified recombinant proteins for their immunogenicity using a T cell proliferation assay as either gel purified protein or a nitrocellulose- bound protein (T cell immunoblot assay) in order to determine whether these antigens elicit T cell responses and finally to isolate T cell clones that elicit strong responses to these antigens and then engraft them to infected SCID mice to determine whether these can reduce parasite load or induce recovery.