FAST ACTION DYNAMICS OF MARIJUANA

尽管在药物治疗中广泛使用尿液中的大麻素分析， 工作场所监测和法医毒物学计划，限制尿液 文献中提供了大麻素排泄数据，以帮助 对测试结果的解释。许多现有的出版作品 信息基于非特异性免疫分析测试的使用， 随着时间的推移，性能特征发生了巨大的变化。另外， 标本采集的时间范围和标本的数量 这些研究中分析的数据都受到了限制。尿液排泄 利用GC/MS研究了大麻烟熏过程中的动力学 本研究收集的尿中大麻素数据。实质性交叉学科 受试者之间和剂量之间的可变性在以下模式中被注意到 11-去甲基-9-羧基-D9-四氢大麻酚(THCCOOH)排泄。平均峰值 尿中四羟甲酸平均浓度分别为89.8+/-31.9 ng/mL和153.4+/- 吸烟约15.8毫克和33.8毫克THC后49.2 ng/毫升， 分别进行了分析。尿液浓度峰值时间平均为7.7h+/- 1.75%THC剂量后0.8，3.55%THC剂量后13.9h+/-3.5。平均 最后一次阳性尿样的GC/MS THCCOOH检测次数 吸一支1.75%或3.55%的香烟后，THC为33.7 分别为+/-9.2h和88.6+/-9.5h 采用浓缩法。 尽管已知大麻代谢物的排泄 在很长一段时间内，很少有研究被设计成 准确估计排泄物的半衰期。我们监测了尿液中 用GC/MS分析大麻的初级尿代谢物THCCOOH 测定了6名男性受试者中THCCOOH的消除半衰期 在吸食一支1.75%或3.55%THC香烟后， 剩余排泄量(ARE)或西格玛减去法 排泄率法。由ARE计算的平均+/-扫描电子显微镜半衰期 方法低剂量或高剂量给药后31.4+/-1.1h(27.8~ 35.3)和28.6+/-1.5h(24.9~34.5)。金额 在七天内排出的THCCOOH为93.9+/-24.5微克(范围 34.6ug至171.6)和197.6+/-33.7ug。这项研究记录了长期的 THCCOOH在尿液中的排泄，强调研究的重要性 终端消除半衰期的精确估计设计。一个 灵敏的分析方法和一到两周的样本采集 周期是大麻监测中的重要研究考虑因素 排泄物。

Despite widespread use of urine cannabinoid assays in drug treatment, workplace monitoring, and forensic toxicology programs, limited urinary cannabinoid excretion data are available in the literature to aid in the interpretation of test results. Much of the available published information is based on the use of non-specific immunoassay tests that have dramatically changed performance characteristics over time. Also, both the timeframe of specimen collection and the number of specimens analyzed in these studies have been restricted. The urinary excretion kinetics of smoked marijuana have been characterized utilizing GC/MS urine cannabinoid data collected in this study. Substantial intersubject variability was noted between subjects and between doses in patterns of 11-nor-9-carboxy-D9-tetrahydrocannabinol (THCCOOH) excretion. Mean peak urine THCCOOH concentrations averaged 89.8 +/- 31.9 ng/mL and 153.4 +/- 49.2 ng/mL following smoking of approximately 15.8 mg and 33.8 mg THC, respectively. The mean times of peak urine concentration were 7.7 h +/- 0.8 after the 1.75% THC and 13.9 h +/- 3.5 after the 3.55% THC dose. Mean GC/MS THCCOOH detection times for the last positive urine sample following the smoking of a single 1.75% or 3.55% THC cigarette were 33.7 +/- 9.2 h and 88.6 +/- 9.5 h, respectively, when a 15 ng/mL cutoff concentration was utilized. Although it is known that the excretion of marijuana metabolites occurs over an extended period of time, few studies have been designed to accurately estimate excretion half-lives. We monitored excretion of the primary urinary metabolite of marijuana, THCCOOH, by GC/MS. Terminal elimination half-lives of THCCOOH were determined in six male subjects following smoking of a single 1.75% or 3.55% THC cigarette by both the amount remaining to be excreted (ARE) or sigma minus method and by the excretion rate method. Mean +/- SEM half-lives calculated by the ARE method following the low or high dose were 31.4 +/- 1.1 h (range 27.8 to 35.3) and 28.6 +/- 1.5 h (range 24.9 to 34.5), respectively. The amounts of THCCOOH excreted over a seven day period were 93.9 +/- 24.5 ug (range 34.6 to 171.6) and 197.6 +/- 33.7 ug. This study documents the prolonged excretion of THCCOOH in urine and emphasizes the importance of study design in precise estimation of terminal elimination half-lives. A sensitive analytical method and a one to two week specimen collection period are important study considerations in the monitoring of marijuana excretion.