ISOLATION AND CHARACTERIZATION OF IMMUNOMODULATING PLANT POLYSACCHARIDES

据报道，几种草药具有刺激性， 对免疫系统有影响，并且相对无毒。 这个项目 进行了分离和表征免疫刺激 中草药多糖的性质 包括：（a）黄芪，（B）蛇床子，（c）甘草， 及（d）Hoelen。 水提法提取多糖，多糖含量为80% 乙醇沉淀，Sephadex G-50凝胶过滤和Sephadex DEAE A-50色谱法。 PS的分子量显示为： 六万一千赫伦两万一千 蛇床子表现出几个小峰， SephadexG-150柱层析中，没有分离峰。 单糖 通过气相色谱法鉴定PS的含量如下： β- Rha、Fuc、Ara、Xyl、Man、Glc和Gal; Hoelen - Fuc、Man， Glc和Gal。 结果表明，从中国人（B）和荷兰人（Hoelen）获得的PS表现出较高的 抗补体活性（未处理对照的16.8-22.8%）。 此外，Hoelen PS表现出细胞因子诱导活性， 干扰素-r和TNF-α的产生。 联合国难民事务高级专员办事处加强了 肺炎球菌PS-蛋白结合物在免疫系统中产生更多的IL-6。

Several herbal medicines have been reported to exhibit stimulating effects on the immune system and are relatively nontoxic. This project was conducted to isolate and characterize the immunostimulating properties of polysaccharides (PSs) from Chinese plant medicines including (a) Astragali radix, (b) Cnidii rhizoma, (c) Angelicae radix, and (d) Hoelen. Polysaccharides were isolated by water extraction, 80% ethanol precipitation, Sephadex G-50 gel filtration and Sephadex DEAE A-50 chromatography. Molecular weights of PSs were shown as; Angelicae 61,000, Hoelen 21,000. Cnidii showed several small peaks while Astragali exhibited no peak in Sephadex G-150 column. Monosaccharide contents of PSs were identified by gas chromatography as follows: Angelicae -- Rha, Fuc, Ara, Xyl, Man, Glc, and Gal; Hoelen -- Fuc, Man, Glc, and Gal. The PSs obtained from Angelicae (B) and Hoelen showed high anticomplementary activity (16.8-22.8% of non-treated control). Furthermore, Hoelen PS exhibited cytokine inducing activity for production of interferon-r and TNF-a. Angelicae PS enhanced the pneumococcal PS-protein conjugate to produce more IL-6 in immune system.