CSHL COURSE--IN SITU HYBRIDIZATION & IMMUNOCYTOCHEMISTRY

描述：（申请人的描述）原位杂交和 免疫细胞化学-1998年10月14日至27日 冷泉港实验室建议继续开设一门名为 “高级原位杂交和免疫细胞化学”，将在 1998年秋季至2003年。 这是一个简短的，为期两周的密集课程， 学生（从研究生到首席研究员）， 直接进入研究，利用先进的和/或专门的 在显微镜技术定位核酸和蛋白质以及 提供这些生物化学概念的背景， 技术. 这些技术将用于表征拷贝数变化 癌基因和抑癌基因的DNA水平，并分析 mRNA和蛋白质的细胞分布和表达状态 与人类致癌相关的（如增殖标记物和肿瘤 抑制基因产物）。 该课程将使学生获得 在定位基因，染色体和mRNA的第一手经验， 荧光原位杂交（FISH），进行光谱核型分析 人类染色体，比较正常与肿瘤样本的基因组 通过比较基因组杂交（CGH），制备抗体制剂， 对于免疫标记，使用不同的抗原-抗体进行实验 系统（荧光，酶，胶体金），以及检查 细胞使用不同的国家的最先进的显微系统（落射荧光 显微镜，共聚焦激光扫描显微镜，透射电子显微镜 显微术）。 该课程由国际公认的领导人授课， 提供实践培训的人。 邀请讲师谁给 关于当前研究的最新报告

DESCRIPTION: (Applicant's Description) Advances In-Situ Hybridization and Immunocytochemistry - October 14-27,1998 The Cold Spring Harbor Laboratory proposes to continue a course entitled "Advanced In Situ Hybridization and Immunocytochemistry," to be held in the Fall of 1998-2003. This is a short, two week, intensive course which trains students (ranging from graduate students to Principal Investigators) to enter directly into research that makes use of advanced and/or specialized techniques in microscopy to localize nucleic acids and proteins as well as to provide background on the biochemical concepts underlying these techniques. The techniques will be used to characterize copy number changes of oncogenes and tumor suppressor genes on the DNA-level, and to analyze the cellular distribution and the expression status of mRNAs and proteins relevant to human carcinogenesis (such as proliferation markers and tumor suppressor gene products). The course will enable students to gain first-hand experience in localizing genes, chromosomes, and mRNAs by fluorescence in situ hybridization (FISH), performing spectral karyotyping of human chromosomes, comparing the genomes of normal versus tumor samples by comparative genomic hybridization (CGH), preparing antibody preparations for immunolabeling, performing experiments using different antigen-antibody systems (fluorescence, enzymatic, colloidal gold), as well as examining cells using different state-of-the-art microscopic systems (epifluorescence microscopy, confocal laser scanning microscopy, transmission electron microscopy). The course is taught by internationally recognized leaders in the field who provide hands-on training. Lecturers are invited who give up-to-the-minute reports on current research.