FLUORESCENT MICROSPHERE COUNTING FOR FLOW MEASUREMENTS

这项研究的长期目标是开发低成本和准确的 组织中局部血流的测量方法。这项研究是基于 关于组织消化中的荧光微球的工作假说 可在荧光流动系统中准确、快速地计数。这个 项目将开发工具和评估程序以进行计数 组织消化液中的微球。在第一阶段中，连续流动 荧光微球计数系统的组装和条件 用于测定微球的准确计数。时间配置文件 将对微球传代进行评估，并将结果与 荧光分光光度法。第一阶段研究将证明 验证工作假设的可行性，并提供设计参数 在H阶段期间建造自动化系统，以便在 生理学研究环境，以评估其实用性和易用性。 该项目将为研究人员提供一个有价值的工具来降低 区域流量测量所涉及的成本和劳动力，并改善 用荧光微球取代老式微球的吸引力 放射性微球法。 拟议的商业应用：这项技术将取代现有的 估计潜在数量更多的微球数量的方法 准确的方法，具有较低的资本、供应和劳动力成本。 潜在市场包括目前正在使用的数百个实验室 荧光微球和大量额外的研究人员 利用替代的放射性和有色微球方法。

The long term goal of this research is to develop low cost and accurate methods for regional flow measurements in tissues. This research is based on the working hypothesis that fluorescent microspheres in tissue digests can be accurately and rapidly counted in a fluorescence flow system. The project will develop instrumentation and evaluate procedures to count microspheres in tissue digests. During Phase I a continuous flow fluorescent microsphere counting system will be assembled and conditions for the accurate counting of microspheres determined. Time profiles of microsphere passage will be evaluated and results compared with the fluorescence spectrophotometry method. Phase I studies will demonstrate the feasibility of the working hypothesis and provide design parameters for automated systems to be constructed during Phase H for testing in physiology research environments to evaluate its utility and ease of use. This project will provide researchers with a valuable tool for lowering the cost and labor involved in regional flow measurements and improve the attraction of using fluorescent microspheres to replace the older radioactive microsphere methods. PROPOSED COMMERCIAL APPLICATION: This technology would replace current methods for estimating numbers of microspheres with a potentially more accurate method that has lower capital, supplies, and labor costs. Potential markets include the hundreds of laboratories currently using fluorescent microspheres and the large number of additional researchers utilizing the alternative radioactive and colored microsphere methods.