ROBUST, ULTRASENSITIVE METHOD FOR VIROLOGY

描述：(改编自调查人员摘要)最近的数据显示 少数病毒粒子通过抗病毒药物治疗存活下来 存活池的一部分整合到宿主DNA中。因此，在那里 需要灵敏和定量的DNA监测方法 存在潜伏的病毒基因组。BioTraces，Inc.已经开发出强大的 基于定量聚合酶链式反应和多光子检测的DNA诊断 技术(MPD)。该提案将开发以肽核酸为基础的 包括HIV-1在内的逆转录病毒的DNA诊断。在此阶段I 建议，将开发MPD/Pd-PP定量分析方法。敏感度 而特异性则可以精确到HIV-1基因组的几个拷贝。在……里面 第二阶段，将测试多探针MPD/PD-PP检测方法。原油 从人类血液中提取的DNA将用于评估。 建议的商业应用：不可用

DESCRIPTION: (adapted from investigator's abstract) Recent data shows a small population of virions survive treatment with antiviral drugs and part of the surviving pool is integrated into host DNA. Therefore, there is need for sensitive and quantitative methods of DNA monitoring for the presence of latent viral genome. Biotraces, Inc. has developed powerful DNA diagnostics based on quantitative PCR and multiphoton detection technology (MPD). The proposal will develop peptide nucleic acid based DNA diagnostics for retroviruses including HIV-1. In this phase I proposal, quantitative MPD/PD-PP assays will be developed. Sensitivity and specificity down to a few copies of HIV-1 genome are expected. In Phase II, a multiprobe MPD/PD-PP detection method will be tested. Crude DNA extracts from human blood will be used for evaluation. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE