OXIDASE RELATED 48K PHOSPHOPROTEIN OF HUMAN NEUTROPHILS

呼吸爆发氧化酶(RBO)是负责 吞噬细胞和B淋巴细胞产生超氧化物。它正处于休眠状态 休眠的细胞，但当细胞被激活时，它就会复活。在这些人中 RBO的组成部分是一种47K(以前为48K)的蛋白质，称为p47Phox 当含有RBO的细胞被激活时，它就会被磷酸化。这个 本工作的目的是确定磷酸化的位置 P47Phox并阐明它们与RBO活性的关系； 识别与p47Phox相互作用的蛋白质，并了解这些 分子水平上的相互作用；并产生大量的 P47Phox用于X射线结晶学结构测定。

The respiratory burst oxidase (RBO) is the enzyme responsible for superoxide production by phagocytes and B lymphocytes. It is dormant in resting cells but comes to life when the cells are activated. Among the components of the RBO is a 47K (formerly 48K) protein known as p47phox that becomes phosphorylated when RBO-containing cells are activated. The aims of the present work are to identify the sites of phosphorylation in p47phox and elucidate their relationship to the activity of the RBO; to identify proteins with which p47phox interacts and understand these interactions at a molecular level; and to produce large quantities of p47phox for structure determination by X- ray crystallography.