PULMONARY EFFECTS OF MACHINING FLUID AEROSOLS

说明：鉴于先前的提案确定， 成分有助于吸入的有害肺部效应 加工流体气溶胶，这项研究计划将测试假设 加工液中的分枝杆菌污染 暴露工人过敏性肺炎。 两例病例报告， 文献记载，职业性接触加工液 气溶胶与过敏性肺炎有关。 近日，八 额外的爆发（即，八个不同的加工厂）， 过敏性肺炎已被确认。 在这8个中的7个中， 爆发，龟分枝杆菌被确定为主要污染物， 每种加工液。 此外，M.龟类多见于 暴露于加工液气溶胶的工人，以及6名 诊断为过敏性肺炎的患者中， ELISA检测抗M.龟类。 此外，还将开展试点工作 实验室最近证明，反复气管滴注 M.龟类可产生过敏性肺炎样组织学改变 小鼠肺部的变化。 综合来看，这些结果具有启发性， 不能证明过敏性肺炎与暴露于M之间存在联系。 海龟污染的加工液气溶胶。 一个新的职业 问题已经浮出水面，其中一种目标微生物，M。Chelonae，还没有 在加工液中常规检测或以前在 过敏性肺炎的发病机制。 自从流行病学研究 通常只能提供关系信息，申请人建议 使用过敏性肺炎的动物模型，以确定是否M。 龟是负责过敏性肺炎的诱导， 机械加工液工人。 虽然间接证据表明M. 龟类与最近爆发的过敏症有关 肺炎，这些拟议的对照实验室研究的结果 在制定控制策略之前需要证明因果关系， 作为适当的生物杀灭剂， 加工液的污染物。

DESCRIPTION: Whereas the previous proposal determined that several components contribute to adverse pulmonary effects produced by inhaled machining fluid aerosols, this research proposal will test the hypothesis that mycobacteria contamination of machining fluids produces hypersensitivity pneumonitis in exposed workers. Two case reports in the literature have documented that occupational exposure to machining fluid aerosols is associated with hypersensitivity pneumonitis. Recently, eight additional outbreaks (i.e., eight different machining plants) of hypersensitivity pneumonitis have been identified. In 7 of these 8 outbreaks, Mycobacterium chelonae was identified as a major contaminant of each machining fluid. Also, antibodies to M. chelonae were more common in workers exposed to machining fluid aerosols, and serum from each of 6 patients diagnosed with hypersensitivity pneumonitis was positive in an ELISA for antibodies to M. chelonae. In addition, pilot work in the laboratory has recently demonstrated that repeated tracheal instillation of M. chelonae can produce hypersensitivity pneumonitis-like histological changes in the mouse lung. Taken together, these results are suggestive but not proof of a link between hypersensitivity pneumonitis and exposure to M. chelonae-contaminated machining fluid aerosols. Thus, a new occupational problem has surfaced in which a targeted microbe, M. chelonae, has not been routinely assayed in machining fluids or previously considered in the pathogenesis of hypersensitivity pneumonitis. Since epidemiologic studies can typically provide only relational information, the applicant proposes to use an animal model of hypersensitivity pneumonitis to determine whether M. chelonae is responsible for the induction of hypersensitivity pneumonitis in machining fluid workers. Although indirect evidence suggests that M. chelonae is involved in the recent outbreaks of hypersensitivity pneumonitis, the findings from these proposed controlled laboratory studies are needed in proving causality prior to instituting control strategies such as the appropriate biocide for eradicating the critical microbial contaminant of machining fluids.