Core--Peptide synthesis

描述：(申请人描述)肽合成设施于1988年12月开始运行。该机构是为了向匹兹堡大学癌症研究所的成员提供研究用的多肽而设立的。该机构由弗朗西斯·芬恩博士领导，他在多肽合成领域拥有多年经验。多肽合成设施占地600平方英尺，位于医学院斯凯夫大厅12楼。建立该设施的设备是用美国国立卫生研究院提供的BRS共享仪器赠款购买的。这包括一台应用生物系统430A合成器，分析和制备规模的高效液相色谱设备。UPCI1997年购买了一台应用生物系统协同合成器，以满足对多肽的日益增长的需求，特别是用于筛选目的的少量多肽。合成、质量控制、提纯和设备维护由操作员负责，操作员自1990年以来一直在该设施工作。他负责生产高纯度的多肽。运营计划是基于这样的假设，即如果由一名有经验的个人负责设备，则操作困难较少。芬恩博士负责监督该设施的运作，排除疑难合成序列的故障，就抗原偶联的最佳序列、纯化的必要性、预测的多肽溶解度以及一般的多肽处理和储存方法咨询研究人员。一个咨询委员会讨论了该机制的管理问题，特别是关于文书的时间分配、建立收费结构和人员任命。

Description: (Applicant's Description) The Peptide Synthesis Facility began operations in December of 1988. The Facility was established for the purpose of providing members of the University of Pittsburgh Cancer Institute with peptides for their research. The Facility is under the direction of Dr. Frances Finn, who has had many years of experience in the field of peptide synthesis. The Peptide Synthesis Facility occupies 600 square feet of space on the 12th floor of Scaife Hall in the School of Medicine. Equipment to establish the Facility was purchased with a BRS shared instrument grant from NIH. This included an Applied Biosystems 430A Synthesizer, equipment of High Performance Liquid Chromatography, both analytical and preparative scale. An Applied Biosystems Synergy Synthesizer was purchased by the UPCI in 1997, to meet the increased demand for peptides, especially small amounts for screening purposes. Synthesis, quality control, purification, and equipment maintenance are the responsibility of the operator, a technician who has been with the Facility since 1990. He is responsible for the producing peptides of a high degree of purity. The operational plan is based on the assumption that fewer operation difficulties occur if a single experienced individual has responsibility for the equipment. Dr. Finn oversees the operation of the facility, troubleshooting difficult synthetic sequences, consulting with investigators as to optimal sequences for antigen coupling, the necessity for purification, predicted solubility of peptides and general peptide handling and storage practices. An Advisory Committee discusses management of the Facility especially in regard to allocation of time on the instrument, establishment of fee structures and personnel appointments.