POSTNATAL DEVELOPMENT OF EXPIRATORY MUSCLE

该候选人是儿科的第三年助理教授， 匹兹堡大学医学院，并有一个坚定的承诺， 学术医学，这一点可以从他的研究努力和对 教学 他一直对呼吸肌在 新生儿出现排便障碍。 他正计划 通过获得新的技能，扩大他的研究学习的广度， 期间的肌肉生物化学和体外肌肉收缩生理学 本申请中提出的培训计划。 这些新技能是 为确保未来独立调查人员的成功， 呼吸肌发育 拟议调查的主要研究目标是确定 出生后收缩、生化和耐力特性的变化 腹外斜肌（腹外斜肌的呼气腹肌） 成熟时的“混合”快和慢颤纤纤维群）。 生化 要进行的评估包括分析这块肌肉的肌球蛋白 收缩蛋白组成，其纤维琥珀酸脱氢酶（SDH） 活性和肌原纤维ATP酶活性。 我们计划将 肌原纤维ATP酶和SDH活性。 将对肋膈进行相同的评估（ “混合”快、慢肌纤维吸气肌控制 种群成熟时），和内侧腓肠肌（一个approximular 肌肉控制的“混合”快和慢颤肌纤维群时， 成熟），以便绘制呼气-吸气，换气-吸气， 和头尾比较。 通过描述这些发展 在呼气和吸气肌肉的属性，我们将加深我们的 了解发展中国家的功能潜力 泵

The candidate is a third year Assistant Professor of pediatrics at the University of Pittsburgh School of Medicine and has a strong commitment to academic medicine as evidenced by his research endeavors and commitment to teaching. He has been interested in the role respiratory muscles play in the development of ventilatory failure in newborns. He is planning to expand the breadth of his research studies by acquiring new skills in muscle biochemistry and in vitro muscle contractile physiology during the training program proposed in the present application. These new skills are necessary to ensure successful future independent investigators of respiratory muscle development. The primary research goal of the proposed investigation is to determine the postnatal changes in the contractile, biochemical, and endurance properties of the external abdominal oblique muscle (an expiratory abdominal muscle of "mixed" fast and slow twitch fiber population when mature). Biochemical assessments to be performed include an analysis of this muscle's myosin contractile protein composition, its fiber succinate dehydrogenase (SDH) activity and its myofibrillar ATPase activity. We plan to correlate endurance properties with myofibrillar ATPase and SDH activities. Identical assessments will be performed on the costal diaphragm (an inspiratory muscle control of "mixed" fast and slow twitch muscle fiber population when mature), and the medial gastrocnemius (an appendicular muscle control of "mixed" fast and slow twitch fiber population when mature) in order to draw expiratory-inspiratory, ventilatory-appendicular, and rostral-caudal comparisons. By characterizing these developmental properties in expiratory and inspiratory muscle, we will deepen our understanding of the functional potential of the developing ventilatory pump.