PROLIFERATION AND FUNCTION OF MONONUCLEAR PHAGOGYTES

小鼠和人单核吞噬细胞的增殖动力学将 被研究。 所需因素之间的关系， 促进增殖和抑制增殖的物质将被 定义了 我们认为，一旦我们了解了扩散是如何 我们将能够在疾病期间改变宿主的环境 动员有效数量的单核吞噬细胞。 不闲聊 我们在多大程度上试图减少病原体或肿瘤的负担， 这些正常宿主防御细胞对抗的负担，它们将是 如果可用的太少，则无效。 我们也在研究 单核吞噬细胞增殖，以提供这些细胞的克隆。 足够多的细胞来研究它们的功能异质性。 使用流式细胞仪和细胞分选仪，我们将研究 单核吞噬细胞增殖。 通过去除生长因子 我们还可以在没有增殖的情况下研究它们的分化。 我们计划研究以下分化标志物： 来自非粘附骨髓祖细胞的后代的粘附， 吞噬作用的发展和膜标志物的发展 Ia、Fc受体和C3b受体。 我们将能够确定从 这些研究是否功能或标记表达的monouclear 吞噬细胞代表所有细胞通过的分化状态， 是否有些功能是克隆起源的，并且是离散的 细胞亚群。

The proliferation kinetics of murine and human mononuclear phagocytes will be studied. The relationship between factors which are required for and promote proliferation and those which inhibit proliferation will be defined. It is our belief that once we understand how proliferation is regulated we will be able to alter the host's environment during disease states to mobilize effective numbers of mononuclear phagocytes. No natter how much we try to reduce the burden of pathogenic organisms or tumor burden which these normal host defense cells combat, they will be ineffective if there are too few available. We are also studying mononuclear phagocyte proliferation in order to provide clones of these cells in large enough numbers to study their functional heterogeneity. Using the Flow Cytometer and Cell Sorter, we will study differentiation of mononuclear phagocytes as they proliferate. By removing the growth factors we can also study their differentiation in the absence of proliferation. We plan to study the following markers of differentiation: The rate of adherence of progeny derived from nonadherent bone marrow progenitor cells, the development of phagocytosis and the development of membrane markers for Ia, Fc receptors and C3b receptors. We will be able to determine from these studies whether functions or markers expressed by monouclear phagocytes represent differentiated states through which all cells pass or whether some functions are of clonal origin and unique to a discrete subpopulation of cells.