Fluorescent sensing of the intracellular redox environment

该项目旨在开发化学方法来测量活细胞的氧化还原环境。氧化和还原（氧化还原）反应对细胞健康和疾病至关重要，已知细胞的氧化还原状态会影响它们对外部应激的反应，但令人惊讶的是，仍然没有有效和准确地确定细胞氧化还原环境的方法。在这个项目中，将设计新的荧光氧化还原传感器，并将其纳入能够对细胞类型进行高通量分析的检测中。这些基础的化学和生化研究可以为未来研究氧化还原环境如何作为疾病和治疗结果的预测因子提供基础。

This project aims to develop chemical approaches to measure the redox environment of living cells. Oxidation and reduction (redox) reactions are crucial for cellular health and disease and the redox status of cells is known to affect their response to external stress, but surprisingly there remains no way of efficiently and accurately determining cellular redox environments. In this project, new fluorescent redox sensors will be designed and incorporated into assays that enable high-throughput analysis of cell types. These fundamental chemical and biochemical studies could provide the basis of future research into how redox environment can be used as a predictor for disease and therapeutic outcomes.