MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION

单核吞噬细胞系统的细胞与广泛的 多种免疫反应相关的功能和活动。 这些包括 参与宿主对肿瘤形成和感染性生物的防御， 以及在免疫应答的阳性和阴性控制中的作用。 的 在这些功能的发展中增强巨噬细胞活性， 调节，至少部分，由可溶性介质期间产生的 免疫反应的过程。 然而，这种机制 控制巨噬细胞功能的因素仍然知之甚少。 的 拟议研究的目的是了解 巨噬细胞活化的分子基础，通过免疫化学 分析细胞表面变化与活化的 巨噬细胞通过干扰素。 使用已建立的巨噬细胞系 免疫，异种单克隆抗体探针将被开发， 通过用以下物质处理在巨噬细胞上诱导的细胞表面抗原 免疫干扰素 这些抗体将用于详细的 不同阶段巨噬细胞表面抗原表型分析 的活化，并鉴定起作用的特异性抗原， 在抗原呈递细胞功能或杀肿瘤活性中 激活细胞 这些抗体也将与以下药物组合使用： 生物化学技术，检测和纯化功能相关基因 用于分子表征的产品 这些抗体最终可能是 在选择性操纵巨噬细胞的实际水平上是有用的 在体内发挥作用。

The cells of the mononuclear phagocyte system are associated with a wide variety of immune response-related functions and activities. These include a participation in host defense against neoplasia and infectious organisms, and a role in the positive and negative control of immune responses. The potentiation of macrophage activity in the development of such functions is regulated, at least in part, by soluble mediators produced during the course of an immune response. However, the mechanisms by which such factors control macrophage function are still poorly understood. The objective of the proposed research is to gain an understanding of the molecular basis of macrophage activation, through the immunochemical analysis of cell surface alterations associated with the activation of macrophages by interferon. Using established macrophage cell lines for immunization, xenogeneic monoclonal antibody probes will be developed to cell surface antigens which are induced on macrophages by treatment with immune interferon. These antibodies will then be used in a detailed analysis of the surface antigen phenotype of macrophages at various stages of activation, and to identification of specific antigens which play a role in the antigen-presenting cell function or tumoricidal activity of activated cells. The antibodies will also be used, in combination with biochemical techniques, to detect and purify functionally relevant gene products for molecular characterization. Such antibodies ultimately may be useful on a practical level in the selective manipulation of macrophage function in vivo.