MONOCLONAL ANTIBODY ANALYSIS OF MACROPHAGE ACTIVATION

单核吞噬细胞系统的细胞与广泛的 各种与免疫反应相关的功能和活动。这些措施包括 参与宿主对肿瘤和传染病的防御， 并在免疫反应的积极和消极控制中发挥作用。这个 巨噬细胞活性的增强在这些功能的发展中是 至少部分地由过程中产生的可溶性介体调节。 免疫反应的进程。然而，这种情况的机制 控制巨噬细胞功能的因素仍然知之甚少。这个 拟议研究的目的是了解 巨噬细胞活化的分子基础，通过免疫化学 与激活相关的细胞表面改变的分析 通过干扰素激活巨噬细胞。利用已建立的巨噬细胞系 免疫后，将开发异种单抗探针以 巨噬细胞上诱导的细胞表面抗原 免疫干扰素。然后这些抗体将被用于详细的 不同发育阶段巨噬细胞表面抗原表型分析 激活，并鉴定起作用的特定抗原 在抗原提呈细胞功能或杀瘤活性方面 激活的细胞。抗体也将被用于与 生化技术，检测和纯化功能相关基因 用于分子表征的产品。这种抗体最终可能是 在实用水平上对巨噬细胞的选择性操作有用 在体内发挥作用。

The cells of the mononuclear phagocyte system are associated with a wide variety of immune response-related functions and activities. These include a participation in host defense against neoplasia and infectious organisms, and a role in the positive and negative control of immune responses. The potentiation of macrophage activity in the development of such functions is regulated, at least in part, by soluble mediators produced during the course of an immune response. However, the mechanisms by which such factors control macrophage function are still poorly understood. The objective of the proposed research is to gain an understanding of the molecular basis of macrophage activation, through the immunochemical analysis of cell surface alterations associated with the activation of macrophages by interferon. Using established macrophage cell lines for immunization, xenogeneic monoclonal antibody probes will be developed to cell surface antigens which are induced on macrophages by treatment with immune interferon. These antibodies will then be used in a detailed analysis of the surface antigen phenotype of macrophages at various stages of activation, and to identification of specific antigens which play a role in the antigen-presenting cell function or tumoricidal activity of activated cells. The antibodies will also be used, in combination with biochemical techniques, to detect and purify functionally relevant gene products for molecular characterization. Such antibodies ultimately may be useful on a practical level in the selective manipulation of macrophage function in vivo.