SYNTHETIC MODELS FOR HEMOCYANIN

提出了将综合模型方法应用于载氧的研究。 蛋白质，血蓝蛋白，以获得对基础的了解 活性中心化学。合成化学涉及到制备 新型苯并咪唑络合铜离子的研究 结构类似于为各种状态设想的结构 血蓝蛋白。完成了对类似物的详细研究 Met-血蓝蛋白的注意力现在将集中在更具挑战性的 氧合血蓝蛋白的问题。重点将放在决定性的 用X射线结晶学和X射线衍射法对晶体材料进行表征 光谱方法(IR、ESR、UV-Vis、共振拉曼)。这些化合物 将提供定义良好的系统，用于测试最近的 电子光谱、振动光谱和EXAFS光谱在血蓝蛋白中的应用。 长期的目标是了解化学和 铜蛋白质的生物化学，特别是那些含铜/氧的蛋白质 互动。铜蛋白的详细分子水平研究 必须是治疗疾病的医学方法的基础 铜(例如，威尔逊氏病)或使用铜螯合物的疗法(例如， 抗关节炎、抗肿瘤药物)。

It is proposed to apply the synthetic model approach to the oxygen-carying protein, hemocyanin, in order to gain an understanding of the fundamental active site chemistry. The synthetic chemistry involves the preparation of new benzimidazole chelating ligands which can hold copper ions in structures similar to those envisaged for the various states of hemocyanin. Having completed a detailed study of analogues of met-hemocyanin attention will now be focused on the more challenging problem of oxyhemocyanin. Emphasis will be placed on definitive characterization of crystalline materials by x-ray crystallography and spectroscopic methods (IR, ESR, UV-VIS, Resonance Raman). The compounds will provide well-defined systems on which to test the validity of recent electronic, vibrational and EXAFS spectroscopy applications to hemocyanin. The long term goal is to gain an understanding of the chemistry and biochemistry of copper proteins, particularly those having copper/dioxygen interactions. A detailed molecular level undertanding of copper proteins must underlie medical approaches to the therapy of disorders involving copper (e.g., Wilson's disease) or therapies using copper chelates (e.g., antiarthritic, antitumor agents).