PHARMACOLOGY OF ANTIRHEUMATIC AGENTS

抗风湿药的药理学

• 批准号: 6305948
• 负责人: BRUCE Neil CRONSTEIN
• 金额: $ 2.1万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6305948
• 关键词: adenosine; antiinflammatory agents; biological signal transduction; clinical research; clinical trials; drug screening /evaluation; enzyme linked immunosorbent assay; human subject; human therapy evaluation; methotrexate; northern blottings; nucleic acid chemical synthesis; polymerase chain reaction; rheumatoid arthritis; skeletal disorder chemotherapy; skeletal pharmacology; sulfanilamides

This study demonstrates that in in vivo models and patients with rheumatoid arthritis the effects of antiinflammatory drugs, specifically methotrexate and sulfasalzine, are due to their capacity to increase extracellular adenosine. The investigators have concluded that signal transduction of adenosine proceeds by a novel pathway. The lab was used for DNA sequencing (automated), ELISA, oligonucleotide synthesis, PCR, ultracentrifugation, and recombinant DNA techniques.

这项研究表明，在体内模型和类风湿性关节炎患者中，抗风湿药物，特别是甲氨蝶呤和柳氮磺胺吡啶的作用是由于它们增加细胞外腺苷的能力。 研究人员得出结论，腺苷的信号转导通过一种新的途径进行。 该实验室用于DNA测序（自动化），ELISA，寡核苷酸合成，PCR，超离心和重组DNA技术。