STUDY OF HUMAN IMMUNE COMPLEX DISEASES

以前的工作中的作用，免疫复合物的发病机制， 系统性红斑狼疮（SLE）、类风湿性关节炎（RA）及相关疾病 混乱将继续下去。 在SLE研究中，主要重点将是 放置在DNA和反天然DNA（nDNA）的表征上， 低分子量血清DNA-抗DNA复合物中的抗体， 与nDNA滴度升高相关的疾病活动期间 抗体和低补体血症。 这些复合物与 低补体血症将通过补体结合研究来确定。 抗原和抗体的唯一性将通过比较来寻求。 结合研究和研究交叉独特型，可能存在于 nDNA抗体。 这些研究不仅可以提供见解， SLE的发病机制，但在使用中具有实际的临床重要性 血清学检测用于SLE患者的管理。 在RA中， 我们的目标是探索类风湿因子在不同的交叉免疫中的作用。 白痴 类风湿因子的发生率和相对量与 这三种已知的杂交独特型将在以下群体中确定： RA患者 这些类风湿因子与 将测定疾病中出现的血清免疫复合物。 在 此外，与这些类风湿因子反应的独特抗原， 将在这些复合物中寻找抗独特型抗体。 还将 确定人类的交叉独特型是否像小鼠一样， 控制 这将通过确定交叉独特型和IgG 已被证明具有类风湿因子的家系中的同种异型， 多个家庭成员。 这些研究可能会为两者提供新的见解 病因和遗传因素。

Previous work on the role of immune complexes in the pathogenesis of systemic loupus erythematosus (SLE), rheumatoid arthritis (RA) and related disorders will be continued. In studies on SLE, primary emphasis will be placed on the characterization of the DNA and anti-native DNA (nDNA) antibodies in low molecular weight serum DNA-anti DNA complexes that occur during disease activity in association with elevated titers of nDNA antibodies and hypocomplementemia. The relationship of these complexes to hypocomplementemia will be determined by complement fixation studies. Uniqueness of both the antigen and antibody will be sought by comparative binding studies and studying cross idiotypes which may be present among the nDNA antibodies. These studies may not only provide insights to the pathogenesis of SLE but are of practical clinical importance in using serologic tests for the management of patients with SLE. In RA the primary goal will be to explore the role of rheumatoid factors with distinct cross idiotypes. The incidence and relative amounts of rheumatoid factor with the three known cross idiotypes will be determined in populations of patients with RA. The relationship of these rheumatoid factors to the serum immune complexes which occur in the disease will be determined. In addition, unique antigens reactive with these rheumatoid factors and anti-idiotype antibody will be sought in these complexes. It will also be determined if cross idiotypes in man, as in the mouse, are under genetic control. This will be done by determining cross idiotypes and IgG allotypes in pedigrees that have been shown to have rheumatoid factors in multiple family members. These studies may provide new insights to both etiologic and genetic factors in RA.