BIOLOGICAL PROPERTIES OF SV40 EARLY PROTEINS

拟议的研究是生物学研究的继续。 SV 40早期蛋白的特性。 在项目前期，我们 证明了已知的SV 40早期蛋白的抗原相关性， T-抗原，检测质膜上大T-多肽的存在 SV 40感染和转化细胞的抗原性差异 细胞核和质膜中的T-多肽之间，表明细胞质 抑制了由SV 40-腺病毒7杂合病毒诱导的SV 40 T抗原的形式 野生型T抗原的核迁移，并描绘了一个差异， 细胞核和细胞质形式的T抗原之间的磷酸化。 它提议 为了进一步表征膜相关T抗原并确定 T-多肽的区域，其赋予所述多肽的各种功能活性。 分子，并控制其运动到特定的细胞内隔室。 膜结合T抗原在TSTA活性中的作用将被定义为： 它与细胞蛋白质，如非病毒T抗原相互作用的能力 和主要组织相容性复合体产物。 将试图 将编码胞质T抗原的突变基因从杂交体中转移 病毒回到SV 40。 这些研究将扩大我们对 病毒诱导的早期蛋白在转化细胞中的生物学功能， 将提供重要的见解肿瘤转化的过程中， 将军

The proposed studies are continuations of investigations into the biological properties of SV40 early proteins. During the previous project period, we demonstrated the antigenic relatedness of the known SV40 early proteins to large T-antigen, detected the presence of large T-polypeptide in the plasma membrane of SV40-infectd and -transformed cells, demonstrated antigenic differences betweem T-polypeptides in nuclei and plasma membranes, showed that a cytoplasmic form of SV40 T-antigen induced by an SV40-adenovirus 7 hybrid virus inhibited the nuclear migration of wild-type T-antigen, and delineated a difference in phosphorylation between nuclear and cytoplasmic forms of T-antigen. It proposed to further characterize the membrane-associated T-antigen and to define the regions of the T-polypeptide which confer various functional activities to the molecule and which govern its movement to specific intracellular compartments. The role of the membrane-bound T-antigen in TSTA activity will be defined, as will its ability to interact with cellular proteins, such as non-viral T-antigen and major histocompatibility complex products. Attempts will be made to transfer the mutated gene which codes for cytoplasmic T-antigen from the hybrid virus back to SV40. These studies will expand our understanding of the biological functions of virus-induced early proteins in transformed cells and will provide important insights into the process of neoplastic transformation in general.