DNA DAMAGE, PROMOTION AND THE PROOXIDANT STATE

提出了一项研究项目，以调查 细胞在致癌过程中的氧化促进剂状态。这个 调查将需要确认、隔离和 DNA寡聚体的损伤特征是 与活性氧物种的影响有关。这些 也将在暴露在培养中的细胞的DNA中寻找损伤 以促进氧化的条件。要使用的单元 调查的是“正常”和“启动”品系的表皮细胞 它们对晋升的反应是不同的。条件和代理 这可能会导致产生活性氧物种，如 作为自氧化剂(如抗坏血酸)或佛波酯(如 PMA)将接受调查，以了解他们的促销能力 转型。这项研究旨在检验这一假设 促进剂通过修饰 基因组。

A research project is proposed for investigating the role of prooxidant states in the cell on the carcinogenesis process. The investigation would entail the identification, isolation and characterization of lesions from DNA oligomers that are associated with the effects of reactive oxygen species. These lesions will be sought also in the DNA of cells exposed in culture to prooxidant conditions. The cells to be employed in the investigation are epidermal cells of "normal" and "initiated" lines which respond differently to promotion. Conditions and agents which may lead to the generation of reactive oxygen species, such as autoxidizing agents (e.g. ascorbic acid) or phorbol esters (e.g. PMA) will be investigated for their ability to promote transformation. The study is designed to test the hypothesis that promoting agents affect carcinogenesis by a modification of the genome.